Cortactin antibody, N-term
|Application Note||Approx. 80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 61.6kDa according to Human NP_005222.2 and of 61.2kDa according to Mouse NP_031829.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paradis et al, J Cell Sci. 2008 Jun 15;121(Pt 12):1965-72; PMID 18495841). Recommended concentration: 0.01-0.03µg/ml. This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).|
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX21374-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Product Description||Goat polyclonal antibody to Cortactin|
|Background||Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.|
|Synonyms||CORTACTIN, oncogene EMS1, CTTN, EMS1, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate), ems1 sequence (mammary tumor and squamous cell carcinomaassociated (p80/85 src substrate), FLJ34459, Cortactin / EMS1|
|Specificity||This antibody is specific for Cortactin / EMS1.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Human, Rat, Dog, Bovine|