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1 .GTX115575 Cpn10 antibody

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Cpn10 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse
  • ★★★☆☆   (  1  ) Publication  ( 1 )
Cat No: GTX115575
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
  • Anti-Cpn10 antibody used in Western Blot (WB). GTX115575
  • Anti-Cpn10 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115575
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
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  • Datasheet
  • Star Review (1)
    Reference (1)
  • Bio-info
  • Protocol & MSDS
  • Application Information: Cpn10 antibody

Catalog Number GTX115575
★★★☆☆
( 1 )
Publication ( 1 )
Product Name Cpn10 antibody
See all Cpn10 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size11 kDa (note) IsoForm (2)
Positive Controlsmouse brain
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Cpn10 antibody

Full Nameheat shock 10kDa protein 1 (chaperonin 10)
BackgroundThis gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. [provided by RefSeq]
SynonymsCPN10 antibody, EPF antibody, GROES antibody, HSP10 antibody, HSPE1 antibody, "10 kDa heat shock protein, mitochondrial antibody", 10 kDa chaperonin antibody, chaperonin 10 antibody, early-pregnancy factor antibody, heat shock 10kD protein 1 (chaperonin 10) antibody, heat shock 10kDa protein 1 (chaperonin 10) antibody
Cellular LocalizationMitochondrion matrix
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenFull length human Cpn10 Recombinant protein.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Zebrafish, Japanese Medaka, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (98%), Zebrafish (80%), Japanese Medaka (83%), Xenopus laevis (88%), Pig (100%), Chicken (87%), Rhesus Monkey (99%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: Cpn10 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Ovarian
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Cpn10 antibody validated data
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
    • GTX115575 ICC/IF Image
      Cpn10 antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:100.
      Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Cpn10 antibody used in Western Blot (WB). GTX115575
    • GTX115575 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse brain
      15% SDS PAGE
      GTX115575 diluted at 1:1000
      Top
  • Anti-Cpn10 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115575
    • GTX115575 IHC-P Image
      Cpn10 antibody detects Cpn10 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded lung carcinoma.
      Cpn10 antibody (GTX115575) dilution: 1:250.
      Top
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
    • GTX115575 ICC/IF Image
      Cpn10 antibody detects Cpn10 protein at cytoplasm by immunofluorescent analysis.
      Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:200.
      Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
    • GTX115575 ICC/IF Image
      Cpn10 antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis.
      Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 15 min.
      Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
    • GTX115575 ICC/IF Image
      Cpn10 antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:100.
      Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Cpn10 antibody used in Western Blot (WB). GTX115575
    • GTX115575 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse brain
      15% SDS PAGE
      GTX115575 diluted at 1:1000
      Top
  • Anti-Cpn10 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115575
    • GTX115575 IHC-P Image
      Cpn10 antibody detects Cpn10 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded lung carcinoma.
      Cpn10 antibody (GTX115575) dilution: 1:250.
      Top
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
    • GTX115575 ICC/IF Image
      Cpn10 antibody detects Cpn10 protein at cytoplasm by immunofluorescent analysis.
      Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:200.
      Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
    • GTX115575 ICC/IF Image
      Cpn10 antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis.
      Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 15 min.
      Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • StarReview
  • Cpn10 antibody

  • Rating : ★★★☆☆ ( Average 3.0 based on 1 users reviews)
  • Western Blot (WB)(1) :★★★☆☆
  • Anonymous StarReview submitted on 24 Sep 2015
  • Score : ★★★☆☆
  • Application Tested : WB
  • Sample Species : Human
  • Sample : HK2 cells
  • Amount used(ug/cells) : 40ug
  • Blocking conditions
  • Blocking Buffer : 3% BSA
  • Incubation Time & Temperature : RT (℃)/1hr
  • Primary antibody
  • Dilution : 1:1000( conc. of dilution )
  • Incubation Time & Temperature : RT (℃)/1hr
Back to Top
  • Reference for Cpn10 antibody
Application References Application Reactivity
Rappa F et al. Comparative analysis of hsp10 and hsp90 expression in healthy mucosa and adenocarcinoma of the large bowel. Anticancer Res 34 (8):4153-9 (2014). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensHSPE13336Q53X54, P61604600141BC023518AAH2351810.93
Homo sapiensHSPE13336Q53X54, P61604600141NM_002157NP_00214810.93

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusHspe115528Q4KL76, Q64433NM_008303NP_03232910.96
Rattus norvegicusHspe125462P97601NM_012966NP_03709810.88
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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