|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||34 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , *U2OS|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||v-crk sarcoma virus CT10 oncogene homolog (avian)-like|
|Product Description||Rabbit Polyclonal antibody to CrkL (v-crk sarcoma virus CT10 oncogene homolog (avian)-like)|
|Background||This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.|
|Synonyms||CRKL antibody, crk-like protein antibody, v-crk sarcoma virus CT10 oncogene homolog (avian)-like antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Xenopus laevis, Rhesus Monkey|
|Predict Reactivity Note||Rat (96%), Zebrafish (83%), Xenopus laevis (87%), Rhesus Monkey (100%)|