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CrkL antibody

Anti-CrkL antibody used in Western Blot (WB). GTX107677
Anti-CrkL antibody used in Western Blot (WB). GTX107677
Anti-CrkL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107677
Anti-CrkL antibody used in Immunoprecipitation (IP). GTX107677
Anti-CrkL antibody used in IHC (Paraffin sections) (IHC-P). GTX107677
Anti-CrkL antibody used in IHC (Paraffin sections) (IHC-P). GTX107677
Anti-CrkL antibody used in IHC (Paraffin sections) (IHC-P). GTX107677

Cat. No. GTX107677

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse brain , 293T , A431 , HeLa , HepG2

Product Note

IP/MS validation was supported by references (PMID:30377387)

Predict Reactivity

Rat, Zebrafish, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950035

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRK like proto-oncogene, adaptor protein

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Database

Research Area

DATA IMAGES

Anti-CrkL antibody used in Western Blot (WB). GTX107677

GTX107677 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CrkL antibody used in Western Blot (WB). GTX107677

GTX107677 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody (GTX107677) diluted at 1:1000.

Anti-CrkL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107677

GTX107677 ICC/IF Image

CrkL antibody detects CrkL protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CrkL protein stained by CrkL antibody (GTX107677) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-CrkL antibody used in Immunoprecipitation (IP). GTX107677

GTX107677 IP Image

Immunoprecipitation of CrkL protein from 293T whole cell extracts using 5 μg of CrkL antibody (GTX107677).
Western blot analysis was performed using CrkL antibody (GTX107677).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CrkL antibody used in IHC (Paraffin sections) (IHC-P). GTX107677

GTX107677 IHC-P Image

CrkL antibody detects CrkL protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
CrkL antibody (GTX107677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CrkL antibody used in IHC (Paraffin sections) (IHC-P). GTX107677

GTX107677 IHC-P Image

CrkL antibody detects CrkL protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
CrkL antibody (GTX107677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CrkL antibody used in IHC (Paraffin sections) (IHC-P). GTX107677

GTX107677 IHC-P Image

CrkL antibody detects CrkL protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CrkL antibody (GTX107677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

CrkL antibody Cat. No. GTX107677

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2020 in Front Mol Biosci. PMID: 33094104
Date : Anonymous submitted on 19-Jan-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : human CrkL transfected HEK293 cells
Amount used : 10μg
Blocking : 2% BSA, 3% non-fat milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane1: HEK293 cells, Lane2: HEK293 cells transfected with pFN21A human CrkL
Date : Anonymous submitted on 17-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169