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Cyclin A2 antibody

KO/KD Orthogonal Overexpression IP/MS
Anti-Cyclin A2 antibody used in Immunoprecipitation (IP). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

Cat. No. GTX103042

Reference 31
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, African green monkey
Package
100 μl ($399),
25 μl ($169)
See all Cyclin A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Neuro2A , PC-12 , IMR32 , SK-N-AS , HeLa(100 μg/ml Bleomycin treatment for 24 hr)

Product Note

IP/MS validation was supported by references (PMID:30377384)

Predict Reactivity

Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949884

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin A2 , CCN1 , CCNA

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin A2 antibody used in Immunoprecipitation (IP). GTX103042

GTX103042 IP Image

Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103042

GTX103042 ICC/IF Image

Cyclin A2 antibody detects Cyclin A2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 stained by Cyclin A2 antibody (GTX103042) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103042

GTX103042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A (GTX103042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2019.PMID: 30719233

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Orthogonal Overexpression

The data was published in the journal Sci Rep in 2020.PMID: 31980707

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

KO/KD Orthogonal

The data was published in the journal Sci Rep in 2020.PMID: 31980707

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2014. PMID: 24393431

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Orthogonal

The data was published in the journal PLoS One in 2014. PMID: 25105411

Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042

GTX103042 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

REFERENCE

Application Reference

Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Gupta RK et al. EMBO J 2022; 41 (22) : e111653 Cyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral?spread.
Application : WB
Reactivity : Human, African green monkey
San-Yuan Chen et al. Oncol Lett 2022; 23 (2) : 61
Application : WB
Reactivity : Human
Zanr? V et al. Int J Mol Sci 2022; 23 (3) Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
Application : WB
Reactivity : Human
Jennifer P Ditano et al. Cell Cycle 2021; 20 (13) : 1308-1319 Activation of CDC25A phosphatase is limited by CDK2/cyclin A-mediated feedback inhibition.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Cyclin A2 antibody Cat. No. GTX103042

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Date : Anonymous submitted on 08-Mar-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC-3, OECM-1, and SAS cells
Amount used : 50μg
Blocking : 5% non-fat milk in TBST, 30°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Date : Anonymous submitted on 31-Jan-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 and HeLa cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:3000, 4°C, 12Hr
Anti-Cyclin A2 antibody used in Western Blot (WB). GTX103042
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 50μg
Blocking : 5% milk, 4°C, overnightHr
Primary Antibody : 1:1500, 25°C, 1Hr
Submit a Review
Application Reference
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Gupta RK et al. EMBO J 2022; 41 (22):e111653 Cyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral?spread.
Application : WB
Reactivity : Human, African green monkey
San-Yuan Chen et al. Oncol Lett 2022; 23 (2):61
Application : WB
Reactivity : Human
Zanr? V et al. Int J Mol Sci 2022; 23 (3) Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
Application : WB
Reactivity : Human
Jennifer P Ditano et al. Cell Cycle 2021; 20 (13):1308-1319 Activation of CDC25A phosphatase is limited by CDK2/cyclin A-mediated feedback inhibition.
Application : WB
Reactivity : Human
Hinds JW et al. ACS Pharmacol Transl Sci 2021; 4 (4):1449-1461 Inhibition of Protein Synthesis Induced by CHK1 Inhibitors Discriminates Sensitive from Resistant Cancer Cells.
Application : WB
Reactivity : Human
Pietil? EA et al. Nat Commun 2021; 12 (1):3904 Co-evolution of matrisome and adaptive adhesion dynamics drives ovarian cancer chemoresistance.
Application : ICC/IF
Reactivity : Human
Javary J et al. Liver Int 2021; 41 (6):1423-1429 Reptin/RUVBL2 is required for hepatocyte proliferation in vivo, liver regeneration and homeostasis.
Fu H et al. Mol Med Rep 2021; 23 (1) CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.
Application : WB
Reactivity : Human
Zhang G et al. Eur J Pharmacol 2020; 887:173584 Targeting DNA and mutant p53 by a naphthalimide derivative, NA20, exhibits selective inhibition in gastric tumorigenesis by blocking mutant p53-EGFR signaling pathway.
Application : WB
Reactivity : Human
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Honda T et al. Sci Rep 2020; 10 (1):1140 PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression.
Application : WB
Reactivity : Mouse
Ogawa M et al. Oncotarget 2019; 10 (3):404-414 Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity.
Yeh HT et al. Eur J Pharmacol 2019; 863:172658 Flavopereirine induces cell cycle arrest and apoptosis via the AKT/p38 MAPK/ERK1/2 signaling pathway in human breast cancer cells.
Application : WB
Reactivity : Human
Chen SY et al. Int J Mol Med 2019; 44 (5):1687-1696 Piperlongumine induces autophagy in biliary cancer cells via?reactive oxygen species-activated Erk signaling pathway.
Application : WB
Reactivity : Human
Liu WL et al. Molecules 2019; 22 (6):pii: E929 Curcumin Inhibits LIN-28A through the Activation of miRNA-98 in the Lung Cancer Cell Line A549.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Huang CY et al. Cells 2018; 7 (12) Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Honda T et al. J Cell Physiol 2018; (Epub) PDZRN3 regulates differentiation of myoblasts into myotubes through transcriptional and posttranslational control of Id2.
Application : WB
Reactivity : Mouse
Liang HH et al. Life Sci 2018; (Epub) Heat shock protein 27 influences the anti-cancer effect of curcumin in colon cancer cells through ROS production and autophagy activation.
Suk FM et al. Sci Rep 2018; 8 (1):2742 ZFP36L1 and ZFP36L2 inhibit cell proliferation in a cyclin D-dependent and p53-independent manner.
Application : WB
Reactivity : Human
Fu H et al. Mol Med Rep 2018; 17 (3):4337-4344 Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes.
Application : WB
Reactivity : Rat
Kokabu S et al. J Biol Chem 2017; (Epub) The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.
Application : WB
Reactivity : Mouse
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
Application : WB
Reactivity : Human
Lin IL et al. Cancer Cell Int 2014; 14 (1):1 The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB.
Li LH et al. PLoS One 2014; 9 (8):e104203 hinokitiol induces dna damage and autophagy followed by cell cycle arrest and senescence in *-resistant lung adenocarcinoma cells.
Application : WB
Reactivity : Human
Chen Y et al. Biochim Biophys Acta 2012; 1822 (4):573-80 The C-terminus of PARK2 is required for its self-interaction, solubility and role in the spindle assembly checkpoint.
Application : WB, IP
Reactivity : Human
Pan J et al. Oncogene 2010; 29 (47):6245-56 Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169