Cyclin D1 antibody [CD1.1]
|Applications||ELISA, FACS, IHC, IHC-Fr, IHC-P, IP, WB|
| ELISA||Assay-dependent dilution
| Immunohistochemistry||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1/1,000-1/1,0000*
| Immunoprecipitation||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Purification Note||From tissue culture supernatant|
|Full Name||cyclin D1|
|Product Description||Mouse monoclonal antibody [CD1.1] to Cyclin D1|
|Background||The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]|
|Synonyms||cyclin D1 Antibody , PRAD1 Antibody , CCND1 Antibody , D11S287E Antibody , BCL1 Antibody , U21B31 Antibody|
|Species Reactivity||Human, Rat|