Cyclin D1 antibody [N1C3]
|Applications||ICC/IF, IP, WB|
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||34 kDa (note)
|Positive Controls||U87-MG , MCF-7 , MDA-MB-231 , *Huh 7 , *H184B5F5/M10 , *BT-474 , *HCC1937 , *Hs578T , *MDA-MB-231 , *MDA-MB-361 , *MDA-MB-453 , *T47D , *ZR-75-1|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||cyclin D1|
|Product Description||Rabbit Polyclonal antibody to Cyclin D1 (cyclin D1)|
|Background||The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]|
|Synonyms||BCL1 antibody, D11S287E antibody, PRAD1 antibody, U21B31 antibody, CCND1 antibody, B-cell CLL/lymphoma 1 antibody, PRAD1 oncogene antibody, G1/S-specific cyclin D1 antibody, B-cell lymphoma 1 protein antibody, BCL-1 oncogene antibody, G1/S-specific cyclin-D1 antibody, cyclin D1 antibody|
|Immunogen||Full length human Cyclin D1 Recombinant protein.|
|Species Reactivity||Human, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Dog, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Mouse (93%), Dog (94%), Chicken (86%), Rhesus Monkey (99%), Bovine (93%)|