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1 .GTX32314 Cyclin E1 (phospho Thr395) antibody

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Cyclin E1 (phospho Thr395) antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman
Cat No: GTX32314
Package Price Qty
100 μlUSD 319.00
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  • Anti-Cyclin E1 (phospho Thr395) antibody used in Western Blot (WB). GTX32314
  • Anti-Cyclin E1 (phospho Thr395) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32314
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  • Datasheet
  • Application Information: Cyclin E1 (phospho Thr395) antibody

Catalog Number GTX32314
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Product Name Cyclin E1 (phospho Thr395) antibody
See all Cyclin E1 products
ApplicationsIHC-P, WB
Application NoteWB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200
Form SuppliedLiquid
PurificationThe antibody was purified by immunogen affinity chromatography.

  • Specifications: Cyclin E1 (phospho Thr395) antibody

Full Namecyclin E1
BackgroundThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]
SynonymsCCNE1 Antibody , pCCNE1 Antibody , cyclin E1 Antibody , CCNE Antibody
SpecificityRecognizes endogenous levels of Cyclin E1 (pT395) protein.
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin E1 (pT395). The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: Cyclin E1 (phospho Thr395) antibody

Storage BufferLiquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Storage InstructionShipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Apoptosis > Metastasis and Invasion > Invasion
  • Cancer > Cell cycle > Cell division
  • Cancer > Cell cycle > Cyclins > Cyclin E
  • Cancer > Type of cancer > Breast > Other
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Cyclin E1 (phospho Thr395) antibody validated data
  • Anti-Cyclin E1 (phospho Thr395) antibody used in Western Blot (WB). GTX32314
    • GTX32314 WB Image
      Western blot analysis of Cyclin E1 (pT395) expression in HepG2 (A); JAR (B) whole cell lysates.
      Top
  • Anti-Cyclin E1 (phospho Thr395) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32314
    • GTX32314 IHC-P Image
      Immunohistochemical analysis of Cyclin E1 (pT395) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
      Top
  • Anti-Cyclin E1 (phospho Thr395) antibody used in Western Blot (WB). GTX32314
    • GTX32314 WB Image
      Western blot analysis of Cyclin E1 (pT395) expression in HepG2 (A); JAR (B) whole cell lysates.
      Top
  • Anti-Cyclin E1 (phospho Thr395) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32314
    • GTX32314 IHC-P Image
      Immunohistochemical analysis of Cyclin E1 (pT395) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
      Top
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