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Cyclophilin E antibody

Anti-Cyclophilin E antibody used in IHC (Paraffin sections) (IHC-P). GTX103662
Anti-Cyclophilin E antibody used in Western Blot (WB). GTX103662
Anti-Cyclophilin E antibody used in IHC (Paraffin sections) (IHC-P). GTX103662

Cat. No. GTX103662

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cyclophilin E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclophilin E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036711

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase E , CYP-33 , CYP33

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclophilin E antibody used in IHC (Paraffin sections) (IHC-P). GTX103662

GTX103662 IHC-P Image

Cyclophilin E antibody detects PPIE protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
Cyclophilin E antibody (GTX103662) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclophilin E antibody used in Western Blot (WB). GTX103662

GTX103662 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX103662 diluted at 1:1000

Anti-Cyclophilin E antibody used in IHC (Paraffin sections) (IHC-P). GTX103662

GTX103662 IHC-P Image

Cyclophilin E antibody detects Cyclophilin E protein at nucleus and cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Cyclophilin E antibody (GTX103662) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169