Cytokeratin 14 antibody [LL002]
|Applications||FACS, ICC/IF, IHC-Fr, IHC-P, WB|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1/200*
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by ion exchange chromatography|
|Purification Note||From tissue culture supernatant|
|Full Name||keratin 14, type I|
|Product Description||Mouse Monoclonal to Human Cytokeratin 14 Sample|
|Background||This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]|
|Synonyms||Cytokeratin14, Cytokeratin 14, K14 NFJ CK14 EBS3 EBS4|
|Immunogen||Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.|
|Species Reactivity||Human, Dog, Pig, Elephant, Lion|