Cytokeratin 18 antibody [DC10]
|Applications||ELISA, FACS, ICC/IF, IHC-P, IP, WB|
|Application Note||For IP and WB: Use at a dilution of 1-2 µg/ml, overnight in 4ºC|
HeLa human cervix carcinoma cell line
MCF-7 human breast adenocarcinoma cell line
Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail).
Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Non-reducing conditions. SDS-PAGE (10% separating gel). For IHC-P: Use at a dilution of 1:10 - 1:100 Positive tissue: epithelial tissue (skin). For ICC: Use at an assay dependent dilution. Positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. For ELISA: Use at an assay dependent dilution. Sandwich ELISA Positive control: MCF-7 human breast adenocarcinoma cell line. Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||WB: HeLa, MCF-7; IHC-P: colon, skin; ICC/IF and ELISA: MCF-7; |
(Please refer to the vial label for the specific concentration)|
|Purification||> 95% (by SDS-PAGE)|
|Purification Note||Purified from ascites by protein-A affinity chromatography|