APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1-2μg/ml |
1-2μg/ml for 30 min at RT |
1-2μg/10⁶ cells |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length KRT8 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CARD2 , CK8 , CYK8 , K2C8 , K8 , KO , KRT8 , keratin 8 , Cytokeratin 8 , Keratin 8
Cellular Localization
Cytoplasm,Nucleus,Nucleoplasm,Nucleus matrix
Background
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Database
Research Area
DATA IMAGES
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GTX34666 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34666 Cytokeratin 8 antibody [K8/383].
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GTX34666 WB Image
WB analysis of A431 cell lysate using GTX34666 Cytokeratin 8 antibody [K8/383].
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GTX34666 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX34666 Cytokeratin 8 antibody [K8/383]. Green : Primary antibody Red : nucleus
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GTX34666 FACS Image
FACS analysis of HeLa cells using GTX34666 Cytokeratin 8 antibody [K8/383]. Blue : Primary antibody Red : Isotype control
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REFERENCE
There are currently no references for Cytokeratin 8 antibody [K8/383] (GTX34666). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 8 antibody [K8/383] (GTX34666). Be the first to share your experience with this product.