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  • D4 GDI antibody [97A1015]

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1 .GTX11927 D4 GDI antibody [97A1015]

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D4 GDI antibody [97A1015]


  • Host / ClonalityMouse Monoclonal
  • Clone Name97A1015
  • ApplicationsELISA, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX11927
Package Price Qty
50 μgUSD 319.00
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  • Anti-D4 GDI antibody [97A1015] used in IHC (Formalin-fixed paraffin-embedded sections). GTX11927
  • Anti-D4 GDI antibody [97A1015] used in ELISA. GTX11927
  • Anti-D4 GDI antibody [97A1015] used in Western Blot (WB). GTX11927
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  • Datasheet

  • Application Information: D4 GDI antibody [97A1015]

Catalog Number GTX11927
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Product Name D4 GDI antibody [97A1015]
See all D4 GDI products
ApplicationsELISA, IHC-P, WB
Application NoteWB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 23 kDa. Optimal dilutions/concentrations should be determined by the end user.
Positive ControlsHL60 cells treated with 10 ng/ml of an anti-Fas mAb
Form SuppliedLiquid
Concentration0.5 mg/ml (Please refer to the vial label for the specific concentration)
PurificationProtein G affinity purified

  • Specifications: D4 GDI antibody [97A1015]

Full NameRho GDP dissociation inhibitor (GDI) beta
BackgroundD4 GDI belongs to the family of Rho GDIs or GDP dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI 1, D4 GDI/RhoGDI2 and Rho GDI3. It inhibits the release of GDP from the Rho proteins, essential for GTP loading and activation of GTPase activity. D4 GDI is located in the cytoplasm and in involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas mediated apoptosis.
SynonymsLy-GDI, D4 GDI, 602843, 397, P52566, RAP1GN1, GDIA2, D4, LyGDI, ARHGDIB, GDID4, Ly GDI
Cellular LocalizationCytoplasmic and Nuclear
HostMouse
ClonalityMonoclonal
Clone Name97A1015
IsotypeIgG1
ImmunogenSynthetic peptide corresponding to amino acid residue(s) in the Fas-induced cleavage site of human D4-GDI.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: D4 GDI antibody [97A1015]

Storage BufferPBS, containing 0.05% BSA and 0.05% sodium azide
Storage InstructionKeep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Lung > Other
  • Cancer > Type of cancer > Prostate cancer
  • Cell Biology > Cell adhesion > Regulation > Negative regulation
  • Cell Biology > Cytoskeleton
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D4 GDI antibody [97A1015] validated data
  • Anti-D4 GDI antibody [97A1015] used in IHC (Formalin-fixed paraffin-embedded sections). GTX11927
    • GTX11927 IHC-P Image
      Immunohistochemical analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and IMG-160A (bottom) at 5 ug/ml
      Top
  • Anti-D4 GDI antibody [97A1015] used in ELISA. GTX11927
    • GTX11927 ELISA Image
      HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the or D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Imgenex's Intracellular Staining Flow Assay Kit . A: IMG-160A which recognizes only cleaved D4-GDI. B: which recognizes both full-length and cleaved.
      Top
  • Anti-D4 GDI antibody [97A1015] used in Western Blot (WB). GTX11927
    • GTX11927 WB Image
      Western blot analysis of D4GDI in Jurkat cells using at 2 ug/ml and IMG-160A at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. IMG-396A detects both the full-length and cleaved forms of D4GDI while IMG-160A specifically detects only the cleaved form of the protein. IMGENEX's goat anti-mouse Ig HRP conjugate (IMGENEX) was used for this test.
      Top
  • Anti-D4 GDI antibody [97A1015] used in IHC (Formalin-fixed paraffin-embedded sections). GTX11927
    • GTX11927 IHC-P Image
      Immunohistochemical analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and IMG-160A (bottom) at 5 ug/ml
      Top
  • Anti-D4 GDI antibody [97A1015] used in ELISA. GTX11927
    • GTX11927 ELISA Image
      HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the or D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Imgenex's Intracellular Staining Flow Assay Kit . A: IMG-160A which recognizes only cleaved D4-GDI. B: which recognizes both full-length and cleaved.
      Top
  • Anti-D4 GDI antibody [97A1015] used in Western Blot (WB). GTX11927
    • GTX11927 WB Image
      Western blot analysis of D4GDI in Jurkat cells using at 2 ug/ml and IMG-160A at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. IMG-396A detects both the full-length and cleaved forms of D4GDI while IMG-160A specifically detects only the cleaved form of the protein. IMGENEX's goat anti-mouse Ig HRP conjugate (IMGENEX) was used for this test.
      Top
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