DAP3 antibody [N1C2]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||46 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||death associated protein 3|
|Product Description||Rabbit Polyclonal antibody to DAP3 (death associated protein 3)|
|Background||Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq]|
|Synonyms||DAP-3 antibody, DKFZp686G12159 antibody, FLJ12817 antibody, MGC126058 antibody, MGC126059 antibody, MRP-S29 antibody, MRPS29 antibody, bMRP-10 antibody, DAP3 antibody, ionizing radiation resistance conferring protein antibody, death-associated protein 3 antibody, mitochondrial 28S ribosomal protein S29 antibody, "28S ribosomal protein S29, mitochondrial antibody", S29mt antibody, death associated protein 3 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human DAP3. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Bovine|
|Predict Reactivity Note||Mouse (90%), Rat (91%), Bovine (91%)|