|Applications||ICC/IF, IHC-P, IP, WB, IB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| Immunoblotting||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||127 kDa (note)
|Positive Controls||293T , HepG2 , NIH-3T3 , mouse brain , PC-12 , Rat2 , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||damage-specific DNA binding protein 1, 127kDa|
|Product Description||Rabbit Polyclonal antibody to DDB1 (damage-specific DNA binding protein 1, 127kDa)|
|Background||This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]|
|Synonyms||DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, DNA damage-binding protein a antibody, XPE-binding factor antibody, UV-DDB 1 antibody, xeroderma pigmentosum group E-complementing protein antibody, UV-damaged DNA-binding protein 1 antibody, DNA damage-binding protein 1 antibody, XAP-1 antibody, HBV X-associated protein 1 antibody, DDB p127 subunit antibody, UV-damaged DNA-binding factor antibody, damage-specific DNA-binding protein 1 antibody, damage-specific DNA binding protein 1, 127kDa antibody|
|Cellular Localization||Cytoplasm , Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Xenopus Tropicalis, Chicken, Bovine|
|Predict Reactivity Note||Xenopus laevis (96%), Xenopus Tropicalis (93%), Chicken (98%), Bovine (100%)|