|Full Name||ArfGAP with SH3 domain, ankyrin repeat and PH domain 2|
|Product Description||Rabbit Polyclonal antibody to DDEF2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2)|
|Background||This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||AMAP2 antibody, CENTB3 antibody, DDEF2 antibody, FLJ42910 antibody, KIAA0400 antibody, PAG3 antibody, PAP antibody, Pap-alpha antibody, SHAG1 antibody, ASAP2 antibody, "centaurin, beta 3 antibody", development and differentiation-enhancing factor 2 antibody, paxillin-associated protein with ARF GAP activity 3 antibody, pyk2 C-terminus-associated protein antibody, PYK2 C terminus-associated protein antibody, "arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 antibody", development and differentiation enhancing factor 2 antibody, "ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 antibody"|
|Cellular Localization||Cytoplasm , Golgi apparatus , Golgi stack membrane , Cell membrane |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human DDEF2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Xenopus laevis (91%)|