DDX1 antibody [N3C2], Internal
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| Immunoprecipitation||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||82 kDa (note)
|Positive Controls||A431 , H1299 , HeLa , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||DEAD (Asp-Glu-Ala-Asp) box polypeptide 1|
|Product Description||Rabbit Polyclonal antibody to DDX1 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 1)|
|Background||DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq]|
|Synonyms||DBP-RB antibody, UKVH5d antibody, DDX1 antibody, ATP-dependent RNA helicase DDX1 antibody, DEAD box protein retinoblastoma antibody, DEAD box protein 1 antibody, DEAD box polypeptide 1 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1 antibody, DEAD box-1 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 antibody|
|Specificity||Knockdown/Knockout validation was supported by references (PMID:26990997)|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human DDX1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (89%), Xenopus laevis (88%), Chicken (93%), Rhesus Monkey (98%), Bovine (98%)|