DDX3 antibody [N3C2], Internal
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||73 kDa (note)
|Positive Controls||U87-MG , SK-N-SH , IMR32 , SK-N-AS , U87MG , U87-MG Cytoplasm , Human ESC|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked|
|Product Description||Rabbit Polyclonal antibody to DDX3 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked)|
|Background||DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq]|
|Synonyms||DBX antibody, DDX14 antibody, DDX3 antibody, HLP2 antibody, DDX3X antibody, helicase like protein 2 antibody, "DEAD box, X isoform antibody", DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3 antibody, CAP-Rf antibody, helicase-like protein 2 antibody, DEAD/H box-3 antibody, ATP-dependent RNA helicase DDX3X antibody, "DEAD box protein 3, X-chromosomal antibody", "DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked antibody"|
|Cellular Localization||Nucleus speckle , Cytoplasm |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Xenopus laevis, Pig, Chicken, Bovine|
|Predict Reactivity Note||Mouse (98%), Xenopus laevis (97%), Pig (100%), Chicken (98%), Bovine (100%)|