DDX4 antibody [C1C3]
|Applications||ICC/IF, IHC-P, WB, IHC-Wm|
Not tested in other applications.
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| IHC (Whole mount)||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||79 kDa (note)
|Positive Controls||NT2D1 , IMR32 , U87-MG , MCF-7|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||DEAD (Asp-Glu-Ala-Asp) box polypeptide 4|
|Product Description||Rabbit Polyclonal antibody to DDX4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4)|
|Background||DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq]|
|Synonyms||MGC111074 antibody, VASA antibody, DDX4 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody, DEAD box protein 4 antibody, vasa homolog antibody, probable ATP-dependent RNA helicase DDX4 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Fish|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Pig, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (90%), Pig (98%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (94%)|