DDX5 antibody [C3], C-term
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| Immunoprecipitation||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||69 kDa (note)
|Positive Controls||A431 , H1299 , HeLa , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||DEAD (Asp-Glu-Ala-Asp) box polypeptide 5|
|Product Description||Rabbit Polyclonal antibody to DDX5 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 5)|
|Background||DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq]|
|Synonyms||DKFZp434E109 antibody, DKFZp686J01190 antibody, G17P1 antibody, HLR1 antibody, HUMP68 antibody, p68 antibody, DDX5 antibody, probable ATP-dependent RNA helicase DDX5 antibody, "DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody", RNA helicase p68 antibody, ATP-dependent RNA helicase DDX5 antibody, DEAD box-5 antibody, DEAD box protein 5 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 antibody|
|Cellular Localization||Nucleus , nucleolus |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDX5. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Dog, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Mouse (100%), Rat (100%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)|