DNA ligase III antibody
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||113 kDa (note)
|Observed Size||92/110 kDa.
The observed M.W. is based on the following publication. PMID:9001252
|Positive Controls||293T , A431 , HeLa , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ligase III, DNA, ATP-dependent|
|Product Description||Rabbit Polyclonal antibody to DNA ligase III (ligase III, DNA, ATP-dependent)|
|Background||This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]|
|Synonyms||LIG2 antibody, LIG3 antibody, ligase II, DNA, ATP-dependent antibody, polydeoxyribonucleotide synthase [ATP] 3 antibody, DNA ligase 3 antibody, ligase III, DNA, ATP-dependent antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human DNA ligase III. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Mouse (92%), Rat (91%), Pig (94%), Rhesus Monkey (97%), Bovine (89%)|