|Applications||EM, ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
| Electron microscopy||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||102 kDa (note)
|Positive Controls||293T , 293T mock , DNMT3A , *CL1-0|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||DNA (cytosine-5-)-methyltransferase 3 alpha|
|Product Description||Rabbit Polyclonal antibody to DNMT3A (DNA (cytosine-5-)-methyltransferase 3 alpha)|
|Background||CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]|
|Synonyms||DNA (cytosine-5-)-methyltransferase 3 alpha Antibody , DNMT3A2 Antibody , M.HsaIIIA Antibody|
|Cellular Localization||Nucleus , Cytoplasm |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3A. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rat (85%), Bovine (81%)|