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  • Daxx antibody [N1N3]

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1 .GTX112908 Daxx antibody [N1N3]

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Daxx antibody [N1N3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX112908
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Daxx antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112908
  • Anti-Daxx antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112908
  • Anti-Daxx antibody [N1N3] used in Western Blot (WB). GTX112908
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  • Datasheet
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  • Application Information: Daxx antibody [N1N3]

Catalog Number GTX112908
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Product Name Daxx antibody [N1N3]
See all Daxx products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size81 kDa (note) IsoForm (3)
Positive ControlsA431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Form SuppliedLiquid
Concentration0.63 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Daxx antibody [N1N3]

Full Namedeath-domain associated protein
BackgroundThis gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
SynonymsBING2 antibody, DAP6 antibody, EAP1 antibody, MGC126245 antibody, MGC126246 antibody, DAXX antibody, CENP-C binding protein antibody, death domain-associated protein 6 antibody, ETS1-associated protein 1 antibody, death-associated protein 6 antibody, Fas-binding protein antibody, fas death domain-associated protein antibody, death-domain associated protein antibody
Cellular LocalizationNucleus , Cytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Daxx. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Bovine
Predict Reactivity NoteMouse (86%), Rat (88%), Bovine (93%)
ConjugationUnconjugated

  • Storage Conditions: Daxx antibody [N1N3]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Apoptosis > Induction > Death domain receptors
  • Cell Biology > Apoptosis > Induction from death receptors
  • Disease Related > Diabetes
  • Immunology > Autoimmunity > Autoimmune diseases > Diabetes
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Daxx antibody [N1N3] validated data
  • Anti-Daxx antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112908
    • GTX112908 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Daxx(GTX112908) antibody at 1:200 dilution.
      Top
  • Anti-Daxx antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112908
    • GTX112908 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Daxx(GTX112908) antibody at 1:500 dilution.
      Top
  • Anti-Daxx antibody [N1N3] used in Western Blot (WB). GTX112908
    • GTX112908 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431
      B: H1299
      C: Raji
      7.5% SDS PAGE
      GTX112908 diluted at 1:1000
      Top
  • Anti-Daxx antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112908
    • GTX112908 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Daxx(GTX112908) antibody at 1:200 dilution.
      Top
  • Anti-Daxx antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112908
    • GTX112908 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Daxx(GTX112908) antibody at 1:500 dilution.
      Top
  • Anti-Daxx antibody [N1N3] used in Western Blot (WB). GTX112908
    • GTX112908 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431
      B: H1299
      C: Raji
      7.5% SDS PAGE
      GTX112908 diluted at 1:1000
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensDAXX1616Q9UER7, Q53F85, Q5STK9, B4E1C1603186NM_001141969NP_00113544181.39
Homo sapiensDAXX1616Q9UER7, Q53F85, Q5STK9, B4E1C1603186NM_001141970NP_00113544282.89
Homo sapiensDAXX1616Q9UER7, Q53F85, Q5STK9, B4E1C1603186NM_001350NP_00134181.39

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Rattus norvegicusDaxx140926Q8VIB2NM_080891NP_54316780.73
Mus musculusDaxx13163Q3UKR0NM_007829NP_03185581.61
Mus musculusDaxx13163Q3UKR0NM_001199733NP_00118666281.61
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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