Dectin-1 antibody [2A11] (Low endotoxin, azide free)
|Applications||FACS, IHC-Fr, IP, Functional Assay|
| IHC (Frozen sections)||Assay-dependent dilution
| Immunoprecipitation||Assay-dependent dilution
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Frozen sections) : The epitope recognized by 2A11 is sensitive to H202 treatment, therefore quenchingwith 0.3% H202 should be performed after incubation with the primary antibody. Theepitope recognized by this antibody is reported to be sensitive to formaldehyde fixation an
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|