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1 .GTX124252 Dengue virus NS3 protein antibody

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Dengue virus NS3 protein antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsFACS, ICC/IF, IHC, IHC-P, WB
  • Species reactivityDengue virus
  • Publication  ( 36 )
Cat No: GTX124252
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
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    Reference (36)
  • Protocol & MSDS

  • Application Information: Dengue virus NS3 protein antibody

Catalog Number GTX124252
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( 0 )
Publication ( 36 )
Product Name Dengue virus NS3 protein antibody
See all Dengue virus NS3 products
ApplicationsFACS, ICC/IF, IHC, IHC-P, WB
Application Note
Suggested dilutionReference
Flow cytometry/FACSAssay-dependent dilution Y
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:2000* Y
Immunohistochemistry (IHC)Assay-dependent dilution Y
IHC (Formalin-fixed paraffin-embedded sections)Assay-dependent dilution Y
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size70 kDa (note)
Positive ControlsDengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21
Form SuppliedLiquid
Concentration0.54 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Dengue virus NS3 protein antibody

Full NameNonstructural protein NS3
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein. The exact sequence is proprietary.
Antigen SpeciesDengue virus
Species ReactivityDengue virus
Cross Reactivity NoteThough raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis.
ConjugationUnconjugated

  • Storage Conditions: Dengue virus NS3 protein antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Infectious Disease > Virus > Dengue Virus (DEV)
More Hide
Dengue virus NS3 protein antibody validated data
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
    • GTX124252 WB Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
      A. 30 µg BHK-21 whole cell extract
      B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
      C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
      D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
      E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
      7.5% SDS-PAGE
      NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      The results may vary from different DENV strains, viral infection status, and culture conditions.
      Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(GTX124252) antibody at 1:2000 dilution.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
    • GTX124252 WB Image
      Sample (20 µg of whole cell lysate)
      A: BHK-21
      B: Dengue virus 2 infect BHK-21
      C: JEV infect BHK-21
      7.5% SDS PAGE
      GTX124252 diluted at 1:2000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
    • GTX124252 WB Image
      Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
      Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
    • GTX124252 WB Image
      NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
      A. 30 µg BHK-21 whole cell extract
      B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
      C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
      D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
      E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
      7.5% SDS-PAGE
      NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      The results may vary from different DENV strains, viral infection status, and culture conditions.
      Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
    • GTX124252 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(GTX124252) antibody at 1:2000 dilution.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
    • GTX124252 WB Image
      Sample (20 µg of whole cell lysate)
      A: BHK-21
      B: Dengue virus 2 infect BHK-21
      C: JEV infect BHK-21
      7.5% SDS PAGE
      GTX124252 diluted at 1:2000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
    • GTX124252 WB Image
      Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.
      Top
Back to Top
  • Reference for Dengue virus NS3 protein antibody
Application References Application Reactivity
Margot Cervantes-Salazar et al. The Nonstructural Proteins 3 And 5 From Flavivirus Modulate Nuclear-Cytoplasmic Transport And Innate Immune Response Targeting Nuclear Proteins. bioRxiv (Epub ahead of print) (2018). WB
Juan Fidel Osuna-Ramos et al. Ezetimibe inhibits Dengue virus infection in Huh 7 cells by blocking the cholesterol transporter Niemann–Pick C1–like 1 receptor. bioRxiv (Epub ahead of print) (2018). WB, FACS
Zhang J et al. Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production. Cell Host Microbe 23 (6):819-831.e5 (2018). WB
Azia S Evans et al. BPIFB3 facilitates flavivirus infection by controlling RETREG1-dependent reticulophagy. bioRxiv 2018 (Epub ahead of print) (2018). ICC/IF
Lai YC et al. Minocycline suppresses dengue virus replication by down-regulation of macrophage migration inhibitory factor-induced autophagy. Antiviral Res 155:28-38 (2018). WB
Sarathy VV et al. Characterization of a murine model of non-lethal, symptomatic dengue virus infection. Sci Rep 8 (1):4900 (2018). IHC-P
McFadden MJ et al. A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time. Viruses 10 (2) (2018). WB
Reid DW et al. Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. J Virol (Epub ahead of print) (2018). WB
Reyes-Ruiz JM et al. Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus. Virology 515:74-80 (2017). ICC/IF
Wu X et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell (Epub ahead of print) (2017). WB
Wan SW et al. Therapeutic Effects of Monoclonal Antibody against Dengue Virus NS1 in a STAT1 Knockout Mouse Model of Dengue Infection. J Immunol (Epub ahead of print) (2017). IHC-P
Lai YC et al. Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection. Sci Rep 7 (1):6975 (2017). IHC-P
Morales L et al. Polyclonal antibodies against recombinant dengue virus NS3 protein. Biomedica 37:1 (2017). ICC/IF, WB
Soto-Acosta R et al. DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog 13 (4):e1006257 (2017). ICC/IF
Soto-Acosta R et al. DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog 13 (4):e1006257 (2017). WB
Bautista-Carbajal P et al. The calmodulin antagonist W-7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride) inhibits DENV infection in Huh-7 cells. Virology 501:188-198 (2017). WB Dengue Virus
Campos RK et al. RPLP1 and RPLP2 are essential flavivirus host factors that promote early viral protein accumulation. J Virol (Epub ahead of print) (2016). WB Dengue Virus
Sun P et al. NK cell degranulation as a marker for measuring antibody-dependent cytotoxicity in neutralizing and non-neutralizing human sera from dengue patients. J Immunol Methods (Epub ahead of print) (2016). FACS
Wu YW et al. Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization. Sci Rep 6:32243 (2016). ICC/IF
Cheng YL et al. Activation of Nrf2 by the dengue virus causes an increase in CLEC5A, which enhances TNF-α production by mononuclear phagocytes. Sci Rep 6:32000 (2016). WB
Tsai TT et al. Microglia retard dengue virus-induced acute viral encephalitis. Sci Rep 6:27670 (2016). IHC-P
Morales L et al. Anticuerpos policlonales contra recombinantes de la proteina NS3 del virus dengue: especificos y versatiles. Biomedica 37:1 (2016).
Marceau C.D. et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535 (7610):159-63 (2016). WB Dengue Virus
Zou J et al. Mapping the Interactions between the NS4B and NS3 proteins of dengue virus. J Virol 89 (7):3471-83 (2015). WB
Medin CL et al. A plasmid-based reporter system for live cell imaging of dengue virus infected cells. J Virol Methods 211:55-62 (2015). ICC/IF
Zou J et al. Characterization of dengue virus NS4A and NS4B protein interaction. J Virol 89 (7):3455-70 (2015). ICC/IF, WB
Yu CY et al. Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins. PLoS Pathog 11 (12):e1005350 (2015).
Chu YT et al. Mast cell-macrophage dynamics in modulation of dengue virus infection in skin. Immunology 146 (1):163-72 (2015). IHC
Naik NG et al. Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication. J Virol 89 (13):6746-60 (2015). WB Dengue virus
Angel-Ambrocio AH et al. An embryonic heart cell line is susceptible to dengue virus infection. Virus Res 198:53-8 (2015). FACS, ICC/IF Dengue virus
Wan SW et al. Protection against dengue virus infection in mice by administration of antibodies against modified nonstructural protein 1. PLoS One 9 (3):e92495 (2014). IHC Dengue virus
Sarathy VV et al. A lethal murine infection model for dengue virus 3 in AG129 mice deficient in Type I and II interferon receptors leads to systemic disease. J Virol (Epub ahead of print) (2014). IHC-P Dengue virus
Soto-Acosta R et al. Nordihydroguaiaretic acid (NDGA) inhibits replication and viral morphogenesis of dengue virus. Antiviral Res 109C:132-140 (2014). WB Dengue virus
St John AL et al. Contributions of mast cells and vasoactive products, leukotrienes and chymase, to dengue virus-induced vascular leakage. Elife 2:e00481 (2013). ICC/IF
Barratt-Boyes SM et al. Comparative immunology, microbiology and infectious diseases. Introduction. Comp Immunol Microbiol Infect Dis 35 (3):217-8 (2012). ICC/IF Dengue virus
Wu CW et al. Method and system for monitoring and recording viral infection process and screening for agents that inhibit virus infection Patent. (2012). ICC/IF Dengue virus
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf

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All products are for research use only—Not for use in diagnostic procedures.