Dengue virus NS3 protein antibody

Host / ClonalityRabbit Polyclonal
ApplicationsELISA, FACS, ICC/IF, IHC, IHC-P, WB, sELISA
Species reactivityDengue virus
( 32 )

Cat No:

GTX124252

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Protocol & MSDS

Application Information: Dengue virus NS3 protein antibody

Catalog Number

GTX124252

Product Name

Dengue virus NS3 protein antibody

See all Dengue virus NS3 products

Applications

ELISA, FACS, ICC/IF, IHC, IHC-P, WB, sELISA

Application Note

	Suggested dilution	Reference
ELISA	1:1000-1:10000*
Flow cytometry/FACS	Assay-dependent dilution	Y
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:2000*	Y
Immunohistochemistry (IHC)	Assay-dependent dilution	Y
IHC (Formalin-fixed paraffin-embedded sections)	Assay-dependent dilution	Y
Western Blot (WB)	1:500-1:3000*	Y
Sandwich ELISA	Assay-dependent dilution

Sandwich ELISA : Capture: GTX124252/GTX629477, Detection: GTX629477/GTX124252.

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

70 kDa (note)

Positive Controls

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

Form Supplied

Liquid

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Dengue virus NS3 protein antibody

Full Name	Nonstructural protein NS3
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein. The exact sequence is proprietary.
Antigen Species	Dengue virus
Species Reactivity	Dengue virus
Cross Reactivity Note	Though raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis.
Conjugation	Unconjugated

Storage Conditions: Dengue virus NS3 protein antibody

Storage Buffer	1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Infectious Disease > Virus > Dengue Virus (DEV) More Hide

Dengue virus NS3 protein antibody validated data

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ELISA Image

ELISA detection of Dengue virus NS3 protein using GTX124252 for capture at a concentration of 5 µg/mL and GTX629477 for detection at a concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10,000 and used to detect the primary antibody.

Top

GTX124252 ELISA Image

ELISA detection of Dengue virus NS3 protein using GTX629477 for capture at a concentration of 5 µg/mL and GTX124252 for detection at a concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10,000 and used to detect the primary antibody.

Top

GTX124252 WB Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

GTX124252 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(GTX124252) antibody at 1:2000 dilution.

Top

GTX124252 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124252 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX124252 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.

Top

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124252 ELISA Image

ELISA detection of Dengue virus NS3 protein using GTX124252 for capture at a concentration of 5 µg/mL and GTX629477 for detection at a concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10,000 and used to detect the primary antibody.

Top

GTX124252 ELISA Image

ELISA detection of Dengue virus NS3 protein using GTX629477 for capture at a concentration of 5 µg/mL and GTX124252 for detection at a concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10,000 and used to detect the primary antibody.

Top

GTX124252 WB Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

GTX124252 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(GTX124252) antibody at 1:2000 dilution.

Top

GTX124252 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124252 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX124252 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.

Top

Reference for Dengue virus NS3 protein antibody

Application References	Application	Reactivity
Lai YC et al. Minocycline suppresses dengue virus replication by down-regulation of macrophage migration inhibitory factor-induced autophagy. Antiviral Res 155:28-38 (2018).	WB
Sarathy VV et al. Characterization of a murine model of non-lethal, symptomatic dengue virus infection. Sci Rep 8 (1):4900 (2018).	IHC-P
McFadden MJ et al. A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time. Viruses 10 (2) (2018).	WB
Reid DW et al. Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. J Virol (Epub ahead of print) (2018).	WB
Reyes-Ruiz JM et al. Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus. Virology 515:74-80 (2017).	ICC/IF
Wu X et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell (Epub ahead of print) (2017).	WB
Wan SW et al. Therapeutic Effects of Monoclonal Antibody against Dengue Virus NS1 in a STAT1 Knockout Mouse Model of Dengue Infection. J Immunol (Epub ahead of print) (2017).	IHC-P
Lai YC et al. Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection. Sci Rep 7 (1):6975 (2017).	IHC-P
Morales L et al. Polyclonal antibodies against recombinant dengue virus NS3 protein. Biomedica 37:1 (2017).	ICC/IF, WB
Soto-Acosta R et al. DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog 13 (4):e1006257 (2017).	ICC/IF
Soto-Acosta R et al. DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog 13 (4):e1006257 (2017).	WB
Bautista-Carbajal P et al. The calmodulin antagonist W-7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride) inhibits DENV infection in Huh-7 cells. Virology 501:188-198 (2017).	WB	Dengue Virus
Campos RK et al. RPLP1 and RPLP2 are essential flavivirus host factors that promote early viral protein accumulation. J Virol (Epub ahead of print) (2016).	WB	Dengue Virus
Sun P et al. NK cell degranulation as a marker for measuring antibody-dependent cytotoxicity in neutralizing and non-neutralizing human sera from dengue patients. J Immunol Methods (Epub ahead of print) (2016).	FACS
Wu YW et al. Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization. Sci Rep 6:32243 (2016).	ICC/IF
Cheng YL et al. Activation of Nrf2 by the dengue virus causes an increase in CLEC5A, which enhances TNF-α production by mononuclear phagocytes. Sci Rep 6:32000 (2016).	WB
Tsai TT et al. Microglia retard dengue virus-induced acute viral encephalitis. Sci Rep 6:27670 (2016).	IHC-P
Morales L et al. Anticuerpos policlonales contra recombinantes de la proteina NS3 del virus dengue: especificos y versatiles. Biomedica 37:1 (2016).
Marceau C.D. et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535 (7610):159-63 (2016).	WB	Dengue Virus
Zou J et al. Mapping the Interactions between the NS4B and NS3 proteins of dengue virus. J Virol 89 (7):3471-83 (2015).	WB
Medin CL et al. A plasmid-based reporter system for live cell imaging of dengue virus infected cells. J Virol Methods 211:55-62 (2015).	ICC/IF
Zou J et al. Characterization of dengue virus NS4A and NS4B protein interaction. J Virol 89 (7):3455-70 (2015).	ICC/IF, WB
Yu CY et al. Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins. PLoS Pathog 11 (12):e1005350 (2015).
Chu YT et al. Mast cell-macrophage dynamics in modulation of dengue virus infection in skin. Immunology 146 (1):163-72 (2015).	IHC
Naik NG et al. Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication. J Virol 89 (13):6746-60 (2015).	WB	Dengue virus
Angel-Ambrocio AH et al. An embryonic heart cell line is susceptible to dengue virus infection. Virus Res 198:53-8 (2015).	FACS, ICC/IF	Dengue virus
Wan SW et al. Protection against dengue virus infection in mice by administration of antibodies against modified nonstructural protein 1. PLoS One 9 (3):e92495 (2014).	IHC	Dengue virus
Sarathy VV et al. A lethal murine infection model for dengue virus 3 in AG129 mice deficient in Type I and II interferon receptors leads to systemic disease. J Virol (Epub ahead of print) (2014).	IHC-P	Dengue virus
Soto-Acosta R et al. Nordihydroguaiaretic acid (NDGA) inhibits replication and viral morphogenesis of dengue virus. Antiviral Res 109C:132-140 (2014).	WB	Dengue virus
St John AL et al. Contributions of mast cells and vasoactive products, leukotrienes and chymase, to dengue virus-induced vascular leakage. Elife 2:e00481 (2013).	ICC/IF
Barratt-Boyes SM et al. Comparative immunology, microbiology and infectious diseases. Introduction. Comp Immunol Microbiol Infect Dis 35 (3):217-8 (2012).	ICC/IF	Dengue virus
Wu CW et al. Method and system for monitoring and recording viral infection process and screening for agents that inhibit virus infection Patent. (2012).	ICC/IF	Dengue virus

*MSDS

MSDS-Glycerol.pdf

MSDS-PBS.pdf

MSDS-ProClin.pdf

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Dengue virus NS3 protein antibody

Dengue virus NS3 protein antibody

Application Information: Dengue virus NS3 protein antibody

Specifications: Dengue virus NS3 protein antibody

Storage Conditions: Dengue virus NS3 protein antibody