Dengue virus prM protein antibody

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, WB
Species reactivityDengue virus
( 4 )

Cat No:

GTX128093

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Reference (4)
Protocol & MSDS

Application Information: Dengue virus prM protein antibody

Catalog Number

GTX128093

Product Name

Dengue virus prM protein antibody

See all Dengue virus prM products

Applications

ICC/IF, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:2000*	Y
Western Blot (WB)	1:1000-1:10000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

22 kDa (note)

Positive Controls

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Dengue virus prM protein antibody

Product Description	Rabbit Polyclonal antibody to prM (Dengue virus)
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Antigen Species	Dengue virus 2
Species Reactivity	Dengue virus
Cross Reactivity Note	Though raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis.
Conjugation	Unconjugated

Storage Conditions: Dengue virus prM protein antibody

Storage Buffer	1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Infectious Disease > Virus > Dengue Virus (DEV) More Hide

Dengue virus prM protein antibody validated data

GTX128093 WB Image

prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
A.30 µg BHK-21 whole cell lysate/extract
B.30 µg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
C.30 µg whole cell lysate/extract of JEV virus infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 WB Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

GTX128093 WB Image

prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
A.30 µg BHK-21 whole cell lysate/extract
B.30 µg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
C.30 µg whole cell lysate/extract of JEV virus infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 WB Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

Reference for Dengue virus prM protein antibody

Application References	Application	Reactivity
Clark KB et al. Characterization of dengue virus 2 growth in megakaryocyte-erythrocyte progenitor cells. Virology 493:162-172 (2016).	WB	Dengue virus
Tham HW et al. Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library. J Infect Dev Ctries 9 (12):1338-49 (2015).	ICC/IF	Dengue virus
Mukherjee S et al. The mechanism and significance of cell type-dependent neutralization of flaviviruses. J Virol (2014).	WB	Dengue virus
VanBlargan LA et al. The type-specific neutralizing antibody response elicited by a dengue vaccine candidate is focused on two amino acids of the envelope protein. PLoS Pathog 9 (12):e1003761 (2013).	WB	Dengue virus

*MSDS

MSDS-Glycerol.pdf

MSDS-PBS.pdf

MSDS-Thimerosal.pdf

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Dengue virus prM protein antibody

Dengue virus prM protein antibody

Application Information: Dengue virus prM protein antibody

Specifications: Dengue virus prM protein antibody

Storage Conditions: Dengue virus prM protein antibody