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1 .GTX128093 Dengue virus prM protein antibody

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Dengue virus prM protein antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityDengue virus
  • Publication  ( 5 )
Cat No: GTX128093
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
  • Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
  • Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
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    Reference (5)
  • Protocol & MSDS
  • Application Information: Dengue virus prM protein antibody

Catalog Number GTX128093
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Publication ( 5 )
Product Name Dengue virus prM protein antibody
See all Dengue virus prM products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:2000* Y
Western Blot (WB)1:1000-1:10000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size22 kDa (note)
Positive ControlsDengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Dengue virus prM protein antibody

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of Dengue virus PrM protein. The exact sequence is proprietary.
Antigen SpeciesDengue virus 2
Species ReactivityDengue virus
Cross Reactivity NoteThough raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis.
ConjugationUnconjugated

  • Storage Conditions: Dengue virus prM protein antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Infectious Disease > Virus > Dengue Virus (DEV)
More Hide
Dengue virus prM protein antibody validated data
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
    • GTX128093 WB Image
      prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
      A.30 µg BHK-21 whole cell lysate/extract
      B.30 µg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
      C.30 µg whole cell lysate/extract of JEV virus infected BHK-21 cells
      15% SDS-PAGE
      prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
    • GTX128093 WB Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
      A. 30 µg BHK-21 whole cell extract
      B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
      C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
      D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
      E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
      15% SDS-PAGE
      prM (Dengue virus) antibody (GTX128093) dilution: 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      The results may vary from different DENV strains, viral infection status, and culture conditions.
      Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.
      Top
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128093
    • GTX128093 ICC/IF Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
      Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
    • GTX128093 WB Image
      prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
      A.30 µg BHK-21 whole cell lysate/extract
      B.30 µg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
      C.30 µg whole cell lysate/extract of JEV virus infected BHK-21 cells
      15% SDS-PAGE
      prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Dengue virus prM protein antibody used in Western Blot (WB). GTX128093
    • GTX128093 WB Image
      prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
      A. 30 µg BHK-21 whole cell extract
      B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
      C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
      D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
      E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
      15% SDS-PAGE
      prM (Dengue virus) antibody (GTX128093) dilution: 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      The results may vary from different DENV strains, viral infection status, and culture conditions.
      Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.
      Top
Back to Top
  • Reference for Dengue virus prM protein antibody
Application References Application Reactivity
Reid DW et al. Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. J Virol (Epub ahead of print) (2018). WB
Clark KB et al. Characterization of dengue virus 2 growth in megakaryocyte-erythrocyte progenitor cells. Virology 493:162-172 (2016). WB Dengue virus
Tham HW et al. Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library. J Infect Dev Ctries 9 (12):1338-49 (2015). ICC/IF Dengue virus
Mukherjee S et al. The mechanism and significance of cell type-dependent neutralization of flaviviruses. J Virol (2014). WB Dengue virus
VanBlargan LA et al. The type-specific neutralizing antibody response elicited by a dengue vaccine candidate is focused on two amino acids of the envelope protein. PLoS Pathog 9 (12):e1003761 (2013). WB Dengue virus
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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