Dengue virus prM protein antibody

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, WB
Species reactivityDengue virus
( 5 )

Cat No:

GTX128093

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Protocol & MSDS

Application Information: Dengue virus prM protein antibody

Catalog Number

GTX128093

Product Name

Dengue virus prM protein antibody

See all Dengue virus prM products

Applications

ICC/IF, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:2000*	Y
Western Blot (WB)	1:1000-1:10000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

22 kDa (note)

Positive Controls

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Dengue virus prM protein antibody

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of Dengue virus PrM protein. The exact sequence is proprietary.
Antigen Species	Dengue virus 2
Species Reactivity	Dengue virus
Cross Reactivity Note	Though raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis.
Conjugation	Unconjugated

Storage Conditions: Dengue virus prM protein antibody

Storage Buffer	1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Infectious Disease > Virus > Dengue Virus (DEV) More Hide

Dengue virus prM protein antibody validated data

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 WB Image

prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
A.30 µg BHK-21 whole cell lysate/extract
B.30 µg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
C.30 µg whole cell lysate/extract of JEV virus infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX128093 WB Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 ICC/IF Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX128093 WB Image

prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
A.30 µg BHK-21 whole cell lysate/extract
B.30 µg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
C.30 µg whole cell lysate/extract of JEV virus infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX128093 WB Image

prM (Dengue virus) antibody detects prM (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

Reference for Dengue virus prM protein antibody

Application References	Application	Reactivity
Reid DW et al. Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. J Virol (Epub ahead of print) (2018).	WB
Clark KB et al. Characterization of dengue virus 2 growth in megakaryocyte-erythrocyte progenitor cells. Virology 493:162-172 (2016).	WB	Dengue virus
Tham HW et al. Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library. J Infect Dev Ctries 9 (12):1338-49 (2015).	ICC/IF	Dengue virus
Mukherjee S et al. The mechanism and significance of cell type-dependent neutralization of flaviviruses. J Virol (2014).	WB	Dengue virus
VanBlargan LA et al. The type-specific neutralizing antibody response elicited by a dengue vaccine candidate is focused on two amino acids of the envelope protein. PLoS Pathog 9 (12):e1003761 (2013).	WB	Dengue virus

*MSDS

MSDS-Glycerol.pdf

MSDS-PBS.pdf

MSDS-Thimerosal.pdf

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Dengue virus prM protein antibody

Dengue virus prM protein antibody

Application Information: Dengue virus prM protein antibody

Specifications: Dengue virus prM protein antibody

Storage Conditions: Dengue virus prM protein antibody