E2F1 antibody [18E10]
|Full Name||E2F transcription factor 1|
|Synonyms||E2F1, E2F-1, Q01094, 189971, 1869, RBP3, RBBP3, RBAP1, E2F 1|
|Product Description||Mouse monoclonal [18E10] antibody to E2F - 1|
|Background||E2F-1-18E10 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry. |
|Immunogen||The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.|
|Applications||ICC/IF, IHC, IP, WB|
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||E2F-1 transfected Soas2 cells|
|Purification||Protein G Affinity Purified|
|Purification Note||From hybridoma tissue culture supernatant|
|Storage Buffer||Phosphate-buffered saline, pH 7.4, containing no preservatives|
|Storage Instruction||Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.|