E2F1 antibody [N2C2], Internal
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||47 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||E2F transcription factor 1|
|Product Description||Rabbit Polyclonal antibody to E2F1 (E2F transcription factor 1)|
|Background||The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq]|
|Synonyms||E2F-1 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, E2F1 antibody, retinoblastoma-binding protein 3 antibody, RBAP-1 antibody, PRB-binding protein E2F-1 antibody, RBBP-3 antibody, transcription factor E2F1 antibody, PBR3 antibody, retinoblastoma-associated protein 1 antibody, E2F transcription factor 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rat (80%), Bovine (86%)|