ECH1 antibody [N1C3]
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||36 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||enoyl CoA hydratase 1, peroxisomal|
|Product Description||Rabbit polyclonal antibody to ECH1 (enoyl Coenzyme A hydratase 1, peroxisomal)|
|Background||This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq]|
|Synonyms||HPXEL antibody, ECH1 antibody, "delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial antibody", "enoyl Coenzyme A hydratase 1, peroxisomal antibody", peroxisomal enoyl-CoA hydratase 1 antibody, "delta3,5-delta2,4-dienoyl-CoA isomerase antibody", dienoyl-CoA isomerase antibody, "enoyl CoA hydratase 1, peroxisomal antibody"|
|Cellular Localization||Mitochondrion , Peroxisome |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rat (82%), Pig (83%)|