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  • EEF1D antibody [N1C3]

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1 .GTX102292 EEF1D antibody [N1C3]

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EEF1D antibody [N1C3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse
Cat No: GTX102292
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292
  • Anti-EEF1D antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102292
  • Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292
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  • Datasheet
  • Bio-info
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  • Application Information: EEF1D antibody [N1C3]

Catalog Number GTX102292
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Product Name EEF1D antibody [N1C3]
See all EEF1D products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size31 kDa (note) IsoForm (10)
Positive ControlsA431 , H1299 , HeLa , HepG2 , mouse brain
Form SuppliedLiquid
Concentration0.91 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: EEF1D antibody [N1C3]

Full Nameeukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
BackgroundThis gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]
SynonymsEF-1D antibody, EF1D antibody, FLJ20897 antibody, FP1047 antibody, EEF1D antibody, antigen NY-CO-4 antibody, elongation factor 1-delta antibody, guanine nucleotide exchange protein antibody, EF-1-delta antibody, eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Rat, Sheep, Bovine
Predict Reactivity NoteRat (92%), Sheep (86%), Bovine (91%)
ConjugationUnconjugated

  • Storage Conditions: EEF1D antibody [N1C3]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
EEF1D antibody [N1C3] validated data
  • Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292
    • GTX102292 WB Image
      Sample (30 ug of whole cell lysate)
      A: H1299
      12% SDS PAGE
      GTX102292 diluted at 1:1000
      Top
  • Anti-EEF1D antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102292
    • GTX102292 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed A431, using EEF1D(GTX102292) antibody at 1:200 dilution.
      Top
  • Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292
    • GTX102292 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX102292 diluted at 1:3000
      Top
  • Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292
    • GTX102292 WB Image
      Sample (30 ug of whole cell lysate)
      A: H1299
      12% SDS PAGE
      GTX102292 diluted at 1:1000
      Top
  • Anti-EEF1D antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102292
    • GTX102292 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed A431, using EEF1D(GTX102292) antibody at 1:200 dilution.
      Top
  • Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292
    • GTX102292 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX102292 diluted at 1:3000
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NP_00127687931.13
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NP_00130467228.56
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NP_00131757528.56
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NM_001130053NP_00112352571.44
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NM_001130055NP_00112352731.13
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NM_001130056NP_00112352828.56
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NM_001130057NP_00112352931.13
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NM_001195203NP_00118213229.08
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NM_001960NP_00195131.13
Homo sapiensEEF1D1936B2RAR6, Q9H7G6, Q4VBZ6, P29692130592NM_032378NP_11575471.44

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusEef1d66656Q68FG5, Q80T06NP_00127236228.73
Mus musculusEef1d66656Q68FG5, Q80T06NP_00127235831.3
Mus musculusEef1d66656Q68FG5, Q80T06NP_00127236028.73
Mus musculusEef1d66656Q68FG5, Q80T06NM_029663NP_08393972.94
Mus musculusEef1d66656Q68FG5, Q80T06NP_00127236330.64
Rattus norvegicusEef1d300033Q68FR9NM_001013104NP_00101312272.14
Mus musculusEef1d66656Q68FG5, Q80T06NM_023240NP_07572931.3
Mus musculusEef1d66656Q68FG5, Q80T06NP_00127236128.73
Mus musculusEef1d66656Q68FG5, Q80T06NP_00127235931.3
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf

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