EGFR antibody [C2C3], C-term
|Applications||FACS, ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
| Flow cytometry/FACS||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||134 kDa (note)
|Observed Size||180 kDa.
|Positive Controls||A431 , *Beas-2 , *A549 , *SiHa , *Hela , *Huh7 , *MSP |
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||epidermal growth factor receptor|
|Product Description||Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor)|
|Background||The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]|
|Synonyms||ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, EGFR antibody, receptor tyrosine-protein kinase erbB-1 antibody, cell growth inhibiting protein 40 antibody, epidermal growth factor receptor antibody, proto-oncogene c-ErbB-1 antibody, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, cell proliferation-inducing protein 61 antibody|
|Cellular Localization||Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rat (87%), Pig (86%)|