EGFR antibody [N1-2], N-term

Host / ClonalityRabbit Polyclonal
ApplicationsFACS, ICC/IF, IHC-P, IP, WB
Species reactivityHuman, Mouse
★★★★☆ ( 1 ) ( 6 )

Cat No:

GTX121919

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Star Review (1)
Reference (6)
Bio-info
Protocol & MSDS

Application Information: EGFR antibody [N1-2], N-term

Catalog Number

GTX121919

Product Name

EGFR antibody [N1-2], N-term

See all EGFR products

Applications

FACS, ICC/IF, IHC-P, IP, WB

Application Note

	Suggested dilution	Reference
Flow cytometry/FACS	Assay-dependent dilution	Y
Immunocytochemistry/ Immunofluorescence (ICC/IF)	Assay-dependent dilution	Y
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Immunoprecipitation	1:100-1:500*	Y
Western Blot (WB)	1:500-1:20000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

134 kDa (note) IsoForm

(8)

Observed Size

180 kDa.

Positive Controls

A431 , A549 , mouse brain , A549 , H1299 , *MSP

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: EGFR antibody [N1-2], N-term

Full Name	epidermal growth factor receptor
Background	The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
Synonyms	ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, EGFR antibody, receptor tyrosine-protein kinase erbB-1 antibody, cell growth inhibiting protein 40 antibody, epidermal growth factor receptor antibody, proto-oncogene c-ErbB-1 antibody, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, cell proliferation-inducing protein 61 antibody
Cellular Localization	Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Rat, Pig
Predict Reactivity Note	Rat (93%), Pig (90%)
Conjugation	Unconjugated

Storage Conditions: EGFR antibody [N1-2], N-term

Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Apoptosis > Metastasis and Invasion > Metastasis > Lymphatic Cancer > Tumor biomarkers Cancer > Type of cancer > Breast > Other Cancer > Type of cancer > Colorectal > Colon More Hide

EGFR antibody [N1-2], N-term validated data

GTX121919 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX121919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR(GTX121919) antibody at 1:500 dilution.

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GTX121919 IHC-P Image

EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis.
Sample: Two independent paraffin-embedded human non-small cell lung cancer.
EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500.

Top

GTX121919 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX121919 IP Image

EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody (GTX121919). The precipitated EGFR protein was detected by GTX121919 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
A: A549 cell lysate.
B: IP by EGFR antibody.
7.5% SDS PAGE

Top

GTX121919 WB Image

Sample (30 µg of whole cell lysate)
A: A431
5% SDS PAGE
GTX121919 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX121919 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX121919 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX121919 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX121919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR(GTX121919) antibody at 1:500 dilution.

Top

GTX121919 IHC-P Image

EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis.
Sample: Two independent paraffin-embedded human non-small cell lung cancer.
EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500.

Top

GTX121919 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX121919 IP Image

EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody (GTX121919). The precipitated EGFR protein was detected by GTX121919 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
A: A549 cell lysate.
B: IP by EGFR antibody.
7.5% SDS PAGE

Top

GTX121919 WB Image

Sample (30 µg of whole cell lysate)
A: A431
5% SDS PAGE
GTX121919 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX121919 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX121919 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

StarReview
EGFR antibody [N1-2], N-term
Rating : ★★★★☆ ( Average 4.0 based on 1 users reviews)
Flow cytometry/FACS(1) :★★★★☆

Anonymous StarReview submitted on 18 Jul 2013
Score : ★★★★☆
Application Tested : Flow
Sample Species : Human
Sample : MSP cells
Blocking conditions
Blocking Buffer : 0.2% FBS in PBS
Incubation Time & Temperature : 4 (℃)/10min
Primary antibody
Dilution : 1:250( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/1hr

Reference for EGFR antibody [N1-2], N-term

Application References	Application	Reactivity
Sasaki N et al. Sialylation regulates myofibroblast differentiation of human skin fibroblasts. Stem Cell Res Ther 8 (1):81 (2017).	FACS	Human
Chen PH et al. The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity. Neuropharmacology (Epub ahead of print) (2016).	WB	Human
Shostak K et al. NF-κB-induced KIAA1199 promotes survival through EGFR signalling. Nat Commun 5:5232 (2014).	IP	Human
Jerman S et al. OFD1 and Flotillins Are Integral Components of a Ciliary Signaling Protein Complex Organized by Polycystins in Renal Epithelia and Odontoblasts. PLoS One 9 (9):e106330 (2014).	ICC/IF	Human
Liu CH et al. Salvianolic Acid B Maintained Stem Cell Pluripotency and Increased Proliferation Rate by Activating Jak2-Stat3 Combined With EGFR-Erk1/2 Pathways. Cell Transplant 23 (4):657-68 (2014).	WB	Mouse
Tsai YC et al. Targeting epidermal growth factor receptor/human epidermal growth factor receptor 2 signalling pathway by a dual receptor tyrosine kinase inhibitor afatinib for radiosensitisation in murine bladder carcinoma. Eur J Cancer 49 (6):1458-66 (2013).	WB	Mouse

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	EGFR	1956	P00533	131550, 211980		NP_001333826	120.71
Homo sapiens	EGFR	1956	P00533	131550, 211980		NP_001333827	125.82
Homo sapiens	EGFR	1956	P00533	131550, 211980		NP_001333828	129.19
Homo sapiens	EGFR	1956	P00533	131550, 211980	BC094761	AAH94761	120.71
Homo sapiens	EGFR	1956	P00533	131550, 211980	NM_005228	NP_005219	134.3
Homo sapiens	EGFR	1956	P00533	131550, 211980	NM_201282	NP_958439	69.24
Homo sapiens	EGFR	1956	P00533	131550, 211980	NM_201283	NP_958440	44.67
Homo sapiens	EGFR	1956	P00533	131550, 211980	NM_201284	NP_958441	77.32

Cross-react Bioinfo
Species	Gene name	Gene ID	SwissProt	Isoform (mRNA)	Protein Accession#	MW
Mus musculus	Egfr	13649	Q01279, Q5SVE8, Q9WVF5	NM_007912	NP_031938	72.92
Mus musculus	Egfr	13649	Q01279, Q5SVE8, Q9WVF5	NM_207655	NP_997538	134.87