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1 .GTX121919 EGFR antibody [N1-2], N-term

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EGFR antibody [N1-2], N-term

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsFACS, ICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Mouse
  • ★★★★☆   (  1  ) Publication  ( 6 )
Cat No: GTX121919
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
  • Anti-EGFR antibody [N1-2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX121919
  • Anti-EGFR antibody [N1-2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX121919
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
  • Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation. GTX121919
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
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  • Datasheet
  • Star Review (1)
    Reference (6)
  • Bio-info
  • Protocol & MSDS
  • Application Information: EGFR antibody [N1-2], N-term

Catalog Number GTX121919
★★★★☆
( 1 )
Publication ( 6 )
Product Name EGFR antibody [N1-2], N-term
See all EGFR products
ApplicationsFACS, ICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Flow cytometry/FACSAssay-dependent dilution Y
Immunocytochemistry/ Immunofluorescence (ICC/IF)Assay-dependent dilution Y
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500* Y
Western Blot (WB)1:500-1:20000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size134 kDa (note) IsoForm (8)
Observed Size180 kDa.
Positive ControlsA431 , A549 , mouse brain , A549 , H1299 , *MSP
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: EGFR antibody [N1-2], N-term

Full Nameepidermal growth factor receptor
BackgroundThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
SynonymsERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, EGFR antibody, receptor tyrosine-protein kinase erbB-1 antibody, cell growth inhibiting protein 40 antibody, epidermal growth factor receptor antibody, proto-oncogene c-ErbB-1 antibody, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, cell proliferation-inducing protein 61 antibody
Cellular LocalizationCell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Pig
Predict Reactivity NoteRat (93%), Pig (90%)
ConjugationUnconjugated

  • Storage Conditions: EGFR antibody [N1-2], N-term

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Apoptosis > Metastasis and Invasion > Metastasis > Lymphatic
  • Cancer > Tumor biomarkers
  • Cancer > Type of cancer > Breast > Other
  • Cancer > Type of cancer > Colorectal > Colon
More Hide
EGFR antibody [N1-2], N-term validated data
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX121919
    • GTX121919 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR(GTX121919) antibody at 1:500 dilution.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX121919
    • GTX121919 IHC-P Image
      EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis.
      Sample: Two independent paraffin-embedded human non-small cell lung cancer.
      EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation. GTX121919
    • GTX121919 IP Image
      EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody (GTX121919). The precipitated EGFR protein was detected by GTX121919 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
      A: A549 cell lysate.
      B: IP by EGFR antibody.
      7.5% SDS PAGE
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Sample (30 µg of whole cell lysate)
      A: A431
      5% SDS PAGE
      GTX121919 diluted at 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      5% SDS PAGE
      GTX121919 diluted at 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX121919
    • GTX121919 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR(GTX121919) antibody at 1:500 dilution.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX121919
    • GTX121919 IHC-P Image
      EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis.
      Sample: Two independent paraffin-embedded human non-small cell lung cancer.
      EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation. GTX121919
    • GTX121919 IP Image
      EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody (GTX121919). The precipitated EGFR protein was detected by GTX121919 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
      A: A549 cell lysate.
      B: IP by EGFR antibody.
      7.5% SDS PAGE
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Sample (30 µg of whole cell lysate)
      A: A431
      5% SDS PAGE
      GTX121919 diluted at 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
    • GTX121919 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      5% SDS PAGE
      GTX121919 diluted at 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • StarReview
  • EGFR antibody [N1-2], N-term

  • Rating : ★★★★☆ ( Average 4.0 based on 1 users reviews)
  • Flow cytometry/FACS(1) :★★★★☆
  • Anonymous StarReview submitted on 18 Jul 2013
  • Score : ★★★★☆
  • Application Tested : Flow
  • Sample Species : Human
  • Sample : MSP cells
  • Blocking conditions
  • Blocking Buffer : 0.2% FBS in PBS
  • Incubation Time & Temperature : 4 (℃)/10min
  • Primary antibody
  • Dilution : 1:250( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/1hr
Back to Top
  • Reference for EGFR antibody [N1-2], N-term
Application References Application Reactivity
Sasaki N et al. Sialylation regulates myofibroblast differentiation of human skin fibroblasts. Stem Cell Res Ther 8 (1):81 (2017). FACS Human
Chen PH et al. The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity. Neuropharmacology (Epub ahead of print) (2016). WB Human
Shostak K et al. NF-κB-induced KIAA1199 promotes survival through EGFR signalling. Nat Commun 5:5232 (2014). IP Human
Jerman S et al. OFD1 and Flotillins Are Integral Components of a Ciliary Signaling Protein Complex Organized by Polycystins in Renal Epithelia and Odontoblasts. PLoS One 9 (9):e106330 (2014). ICC/IF Human
Liu CH et al. Salvianolic Acid B Maintained Stem Cell Pluripotency and Increased Proliferation Rate by Activating Jak2-Stat3 Combined With EGFR-Erk1/2 Pathways. Cell Transplant 23 (4):657-68 (2014). WB Mouse
Tsai YC et al. Targeting epidermal growth factor receptor/human epidermal growth factor receptor 2 signalling pathway by a dual receptor tyrosine kinase inhibitor afatinib for radiosensitisation in murine bladder carcinoma. Eur J Cancer 49 (6):1458-66 (2013). WB Mouse
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensEGFR1956P00533131550, 211980NP_001333826120.71
Homo sapiensEGFR1956P00533131550, 211980NP_001333827125.82
Homo sapiensEGFR1956P00533131550, 211980NP_001333828129.19
Homo sapiensEGFR1956P00533131550, 211980BC094761AAH94761120.71
Homo sapiensEGFR1956P00533131550, 211980NM_005228NP_005219134.3
Homo sapiensEGFR1956P00533131550, 211980NM_201282NP_95843969.24
Homo sapiensEGFR1956P00533131550, 211980NM_201283NP_95844044.67
Homo sapiensEGFR1956P00533131550, 211980NM_201284NP_95844177.32

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusEgfr13649Q01279, Q5SVE8, Q9WVF5NM_007912NP_03193872.92
Mus musculusEgfr13649Q01279, Q5SVE8, Q9WVF5NM_207655NP_997538134.87
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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