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Catalog Number
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GTX121919
(
6
)
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Product Name
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EGFR antibody [N1-2], N-term
|
| Applications | FACS, ICC/IF, IHC-P, IP, WB |
| Application Note |
| Suggested dilution | Reference |
| Flow cytometry/FACS | Assay-dependent dilution |
Y |
| Immunocytochemistry/ Immunofluorescence (ICC/IF) | Assay-dependent dilution |
Y |
| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
|
| Immunoprecipitation | 1:100-1:500* |
Y |
| Western Blot (WB) | 1:500-1:20000* |
Y |
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Predicted Target Size | 134 kDa (note)
 (8)
|
| Observed Size | 180 kDa.
|
| Positive Controls | A431 , A549 , mouse brain , A549 , H1299 , *MSP |
| Form Supplied | Liquid |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | epidermal growth factor receptor |
| Product Description | Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor) |
| Background | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq] |
| Synonyms | ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, EGFR antibody, receptor tyrosine-protein kinase erbB-1 antibody, cell growth inhibiting protein 40 antibody, epidermal growth factor receptor antibody, proto-oncogene c-ErbB-1 antibody, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, cell proliferation-inducing protein 61 antibody |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Rat, Pig |
| Predict Reactivity Note | Rat (93%), Pig (90%) |
| Conjugation | Unconjugated |