|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||17 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||eukaryotic translation initiation factor 5A|
|Product Description||Rabbit Polyclonal antibody to EIF5A (eukaryotic translation initiation factor 5A)|
|Background||mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of TP53/p53 and TP53/p53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.|
|Synonyms||EIF-5A antibody, EIF5A1 antibody, MGC104255 antibody, MGC99547 antibody, eIF5AI antibody, EIF5A antibody, eIF-5A-1 antibody, eukaryotic initiation factor 5A antibody, eukaryotic translation initiation factor 5A-1 antibody, eIF-4D antibody, eIF-5A1 antibody, rev-binding factor antibody, eukaryotic translation initiation factor 5A antibody|
|Cellular Localization||Cytoplasm , Nucleus , Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , nuclear pore complex |
|Immunogen||Full length human EIF5A Recombinant protein.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Sheep, Bovine|
|Predict Reactivity Note||Zebrafish (82%), Xenopus laevis (83%), Sheep (100%), Bovine (100%)|