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Catalog Number
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GTX27183
(
0
)
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Product Name
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EL4 lysate
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| ProductDescription | EL4 whole cell lysate |
| Background | Mouse EL4 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. |
| Lysate Fraction | Whole Cell Extract/Total Protein |
| Cell Lines | EL4 |
| Applications | WB |
| Application Note | EL4 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 μg per lane is recommended for mini gel. |
| Purification | Whole Cell Lysate |
| Concentration | 2
mg/ml
|
| Storage Buffer | Whole cell lysate in 1 X SDS-PAGE sample loading buffer, with 5% b-mercaptoethanol |
| Storage Instruction | Upon receipt - Store at -20ºC short term. Keep as concentrated solution. For extended storage aliquot and store at -70ºC or below. Avoid freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |