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EML1 antibody [C3], C-term

Anti-EML1 antibody [C3], C-term used in Western Blot (WB). GTX100252
Anti-EML1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100252
Anti-EML1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100252
Anti-EML1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100252
Anti-EML1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100252
Anti-EML1 antibody [C3], C-term used in Western Blot (WB). GTX100252

Cat. No. GTX100252

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)
See all EML1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

H1299 , NIH-3T3

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EML1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EMAP like 1 , BH , ELP79 , EMAP , EMAPL , HuEMAP

Cellular Localization

Cytoplasm

Background

Human echinoderm microtubule-associated protein-like is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EML1 antibody [C3], C-term used in Western Blot (WB). GTX100252

GTX100252 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
EML1 antibody
GTX100252 diluted at 1:500

Anti-EML1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100252

GTX100252 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using EML1(GTX100252) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EML1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100252

GTX100252 ICC/IF Image

EML1 antibody [c3], c-term detects EML1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EML1 protein stained by EML1 antibody [c3], c-term (GTX100252) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-EML1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100252

GTX100252 IHC-P Image

EML1 antibody [C3], C-term detects EML1 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
EML1 antibody [C3], C-term (GTX100252) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EML1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100252

GTX100252 IHC-P Image

EML1 antibody [C3], C-term detects EML1 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
EML1 antibody [C3], C-term (GTX100252) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EML1 antibody [C3], C-term used in Western Blot (WB). GTX100252

GTX100252 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX100252 diluted at 1:1000

REFERENCE

Application Reference

Kielar M et al. Nat Neurosci 2014; 17 (7) : 923-33 Mutations in Eml1 lead to ectopic progenitors and neuronal heterotopia in mouse and human.
Application : ICC/IF
Reactivity : Monkey
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169