ENO1 antibody [N3C3]
|Applications||Dot, ICC/IF, IHC-P, WB|
Not tested in other applications.
| Dot blot||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||47 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||enolase 1, (alpha)|
|Product Description||Rabbit Polyclonal antibody to ENO1 (enolase 1, (alpha))|
|Background||This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]|
|Synonyms||ENO1L1 antibody, MPB1 antibody, NNE antibody, PPH antibody, ENO1 antibody, non-neural enolase antibody, 2-phospho-D-glycerate hydro-lyase antibody, alpha-enolase, c-myc promoter-binding protein-1 antibody, alpha enolase like 1 antibody, tau-crystallin antibody, alpha-enolase antibody, MYC promoter-binding protein 1 antibody, phosphopyruvate hydratase antibody, plasminogen-binding protein antibody, enolase-alpha antibody, "enolase 1, (alpha) antibody"|
|Cellular Localization||Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, Bovine, Guinea pig|
|Predict Reactivity Note||Rat (93%), Xenopus laevis (90%), Chicken (93%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (93%), Guinea pig (95%)|