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ERCC1 antibody [1B3]

Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546
Anti-ERCC1 antibody [1B3] used in Flow cytometry (FACS). GTX84546
Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546
Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546
Anti-ERCC1 antibody [1B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84546
Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546
Anti-ERCC1 antibody [1B3] used in Flow cytometry (FACS). GTX84546
Anti-ERCC1 antibody [1B3] used in Western Blot (WB). GTX84546

Cat. No. GTX84546

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all ERCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ERCC1 (NP_001974) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 1, endonuclease non-catalytic subunit , COFS4 , RAD10 , UV20

Cellular Localization

Nucleus

Background

Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.

Database

Research Area

DATA IMAGES

Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546

GTX84546 IHC-P Image

IHC-P analysis of colon tissue using GTX84546 ERCC1 antibody [1B3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERCC1 antibody [1B3] used in Flow cytometry (FACS). GTX84546

GTX84546 FACS Image

FACS analysis of Jurkat cells using GTX84546 ERCC1 antibody [1B3].
Red : Primary antibody
Blue : Negative control antibody
Dilution : 1:100

Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546

GTX84546 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX84546 ERCC1 antibody [1B3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546

GTX84546 IHC-P Image

IHC-P analysis of prostate tissue using GTX84546 ERCC1 antibody [1B3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERCC1 antibody [1B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84546

GTX84546 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ERCC1 plasmid using GTX84546 ERCC1 antibody [1B3].

Anti-ERCC1 antibody [1B3] used in IHC (Paraffin sections) (IHC-P). GTX84546

GTX84546 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84546 ERCC1 antibody [1B3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERCC1 antibody [1B3] used in Flow cytometry (FACS). GTX84546

GTX84546 FACS Image

FACS analysis of HEK293T cells transfected with either ERCC1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84546 ERCC1 antibody [1B3].

Anti-ERCC1 antibody [1B3] used in Western Blot (WB). GTX84546

GTX84546 WB Image

WB analysis of HEK293T cells transfected with ERCC1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84546 ERCC1 antibody [1B3].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519