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ERCC1 antibody [N1C3]

Anti-ERCC1 antibody [N1C3] used in Western Blot (WB). GTX110562
Anti-ERCC1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110562

Cat. No. GTX110562

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729579

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 1, endonuclease non-catalytic subunit , COFS4 , RAD10 , UV20

Background

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERCC1 antibody [N1C3] used in Western Blot (WB). GTX110562

GTX110562 WB Image

Wild-type (WT) and ERCC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC1 antibody [N1C3] (GTX110562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERCC1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110562

GTX110562 ICC/IF Image

ERCC1 antibody [N1C3] detects ERCC1 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERCC1 protein stained by ERCC1 antibody [N1C3] (GTX110562) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Pan CH et al. Chem Biol Interact 2020; 332 : 109304
Application : WB
Reactivity : Human

REVIEW

ERCC1 antibody [N1C3] Cat. No. GTX110562

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 12-Feb-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20 μg
Blocking : 0.3% milk in 0.5% PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Untreated cells, Lane 2: HeLa whole cell lysate treated with si-XPF.
Date : Anonymous submitted on 15-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : KOSC3 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Application Reference
Pan CH et al. Chem Biol Interact 2020; 332:109304
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169