|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||57 kDa (note)
|Positive Controls||HepG2 , HCT116 , 293T , A431 , HeLa , Neuro2A , GL261 , C8D30 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||protein disulfide isomerase family A, member 3|
|Product Description||Rabbit Polyclonal antibody to ERp57 (protein disulfide isomerase family A, member 3)|
|Background||This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]|
|Synonyms||ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PI-PLC antibody, PDIA3 antibody, protein disulfide isomerase-associated 3 antibody, ER protein 60 antibody, 58 kDa glucose-regulated protein antibody, endoplasmic reticulum resident protein 60 antibody, phospholipase C-alpha antibody, ER protein 57 antibody, endoplasmic reticulum P58 antibody, "glucose regulated protein, 58kDa antibody", disulfide isomerase ER-60 antibody, endoplasmic reticulum resident protein 57 antibody, protein disulfide-isomerase A3 antibody, 58 kDa microsomal protein antibody, "protein disulfide isomerase family A, member 3 antibody"|
|Cellular Localization||Endoplasmic reticulum lumen (By similarity) , Melanosome |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Pig (94%), Rabbit (94%), Chicken (80%), Sheep (91%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (93%)|