|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||35 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , C8D30 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||electron-transfer-flavoprotein, alpha polypeptide|
|Product Description||Rabbit Polyclonal antibody to ETFA (electron-transfer-flavoprotein, alpha polypeptide)|
|Background||ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||EMA antibody, GA2 antibody, MADD antibody, ETFA antibody, electron transfer flavoprotein, alpha polypeptide antibody, electron transfer flavoprotein alpha-subunit antibody, electron transfer flavoprotein subunit alpha, mitochondrial antibody, alpha-ETF antibody, glutaric aciduria II antibody, electron-transfer-flavoprotein, alpha polypeptide antibody|
|Cellular Localization||Mitochondrion matrix|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Cat, Pig, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (84%), Xenopus laevis (85%), Cat (96%), Pig (96%), Chicken (87%), Rhesus Monkey (98%), Bovine (96%)|