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1 .GTX32411 BMX (phospho Tyr566) antibody

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BMX (phospho Tyr566) antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX32411
Package Price Qty
100 μlUSD 319.00
Add to cart
 
  • Anti-BMX (phospho Tyr566) antibody used in Western Blot (WB). GTX32411
  • Anti-BMX (phospho Tyr566) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32411
  • Anti-BMX (phospho Tyr566) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32411
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  • Datasheet
  • Application Information: BMX (phospho Tyr566) antibody

Catalog Number GTX32411
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Product Name BMX (phospho Tyr566) antibody
See all BMX products
ApplicationsICC/IF, IHC-P, WB
Application NoteWB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500
Form SuppliedLiquid
PurificationThe antibody was purified by immunogen affinity chromatography.

  • Specifications: BMX (phospho Tyr566) antibody

Full NameBMX non-receptor tyrosine kinase
BackgroundThis gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Sep 2009]
SynonymsPSCTK3 Antibody , ETK Antibody , BMX Antibody , PSCTK2 Antibody , BMX non-receptor tyrosine kinase Antibody
SpecificityRecognizes endogenous levels of BMX (pY566) protein.
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BMX (phospho Tyr566). The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: BMX (phospho Tyr566) antibody

Storage BufferLiquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Storage InstructionShipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
BMX (phospho Tyr566) antibody validated data
  • Anti-BMX (phospho Tyr566) antibody used in Western Blot (WB). GTX32411
    • GTX32411 WB Image
      Western blot analysis of BMX (pY566) expression in HeLa (A) whole cell lysates.
      Top
  • Anti-BMX (phospho Tyr566) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32411
    • GTX32411 IHC-P Image
      Immunohistochemical analysis of BMX (pY566) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
      Top
  • Anti-BMX (phospho Tyr566) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32411
    • GTX32411 ICC/IF Image
      Immunofluorescent analysis of BMX (pY566) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
      Top
  • Anti-BMX (phospho Tyr566) antibody used in Western Blot (WB). GTX32411
    • GTX32411 WB Image
      Western blot analysis of BMX (pY566) expression in HeLa (A) whole cell lysates.
      Top
  • Anti-BMX (phospho Tyr566) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32411
    • GTX32411 IHC-P Image
      Immunohistochemical analysis of BMX (pY566) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
      Top
  • Anti-BMX (phospho Tyr566) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32411
    • GTX32411 ICC/IF Image
      Immunofluorescent analysis of BMX (pY566) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
      Top
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