BMX (phospho Tyr566) antibody

Cat No:

GTX32411

Package	Price	Qty
100 μl	USD 319.00

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Catalog Number	GTX32411 ☆☆☆☆☆ ( 0 ) ( 0 )
Product Name	BMX (phospho Tyr566) antibody See all BMX products
Applications	ICC/IF, IHC-P, WB
Application Note	WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500
Form Supplied	Liquid
Purification	The antibody was purified by immunogen affinity chromatography.

Full Name	BMX non-receptor tyrosine kinase
Background	This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Sep 2009]
Synonyms	PSCTK3 Antibody , ETK Antibody , BMX Antibody , PSCTK2 Antibody , BMX non-receptor tyrosine kinase Antibody
Specificity	Recognizes endogenous levels of BMX (pY566) protein.
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BMX (phospho Tyr566). The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human
Conjugation	Unconjugated

Storage Buffer	Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Storage Instruction	Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

BMX (phospho Tyr566) antibody validated data

GTX32411 WB Image

Western blot analysis of BMX (pY566) expression in HeLa (A) whole cell lysates.

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GTX32411 IHC-P Image

Immunohistochemical analysis of BMX (pY566) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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GTX32411 ICC/IF Image

Immunofluorescent analysis of BMX (pY566) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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GTX32411 WB Image

Western blot analysis of BMX (pY566) expression in HeLa (A) whole cell lysates.

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GTX32411 IHC-P Image

Immunohistochemical analysis of BMX (pY566) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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GTX32411 ICC/IF Image

Immunofluorescent analysis of BMX (pY566) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Primary Antibodies