GeneTex
  • Country / Location Selection

United States (US)

EasyBlot anti-Rat IgG Kit (Optimized for Prot A/G)

Cat. No. GTX228909-01

Application

WB

Reactivity

Rat
Package
250 μl ($399),
50 μl ($169)
PRODUCT

Summary

EasyBlot anti-Rat IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rat IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP).

The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.

Content

EasyBlot Kit Package Content Package Storage Temp
GTX228909-01 (250μl) GTX628474-01 EasyBlot anti Rat IgG (HRP) 250μl -20 ºC
GTX425858 EasyBlocker 60g 4 ºC
GTX228909-01 (50μl) GTX628474-01 EasyBlot anti Rat IgG (HRP) 50μl -20 ºC
GTX425858 EasyBlocker 12g 4 ºC

APPLICATION

Application Note

Suggested starting dilutions for WB is 1:1000. Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

PROPERTIES

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Target Isotype

IgG

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

REFERENCE

There are currently no references for EasyBlot anti-Rat IgG Kit (Optimized for Prot A/G) (GTX228909-01). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EasyBlot anti-Rat IgG Kit (Optimized for Prot A/G) (GTX228909-01). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169