EasyBlot anti-Sheep IgG Kit (Optimized for Prot A/G)
|Background||EasyBlot anti-Sheep IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). |
The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.
Note: Suggested dilution is 1:1000. Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples.
|Application Note||Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|Positive Controls||0.2 µg native sheep IgG , 0.1 µg native sheep IgG|
|Purification||Affinity purified by Protein G.|
(Please refer to the vial label for the specific concentration)|
|Storage Buffer||0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA, 40%glycerol. 0.005% Thimerosal was added as a preservative.|
|Storage Instruction||Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|