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Endothelin A Receptor antibody [C3], C-term

Anti-Endothelin A Receptor antibody [C3], C-term used in Western Blot (WB). GTX116034
Anti-Endothelin A Receptor antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116034
Anti-Endothelin A Receptor antibody [C3], C-term used in Western Blot (WB). GTX116034

Cat. No. GTX116034

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Chicken
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HUVEC

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Endothelin A Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036824

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endothelin receptor type A , ET-A , ETA , ETA-R , ETAR , ETRA , MFDA , hET-AR

Cellular Localization

Cell membrane

Background

This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Endothelin A Receptor antibody [C3], C-term used in Western Blot (WB). GTX116034

GTX116034 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin A Receptor antibody [C3], C-term (GTX116034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Endothelin A Receptor antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116034

GTX116034 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Endothelin A Receptor (GTX116034) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Endothelin A Receptor antibody [C3], C-term used in Western Blot (WB). GTX116034

GTX116034 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin A Receptor antibody [C3], C-term (GTX116034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169