|Application Note||Recommended Starting Dilutions:|
For ICC/IF: Use at 1:10-1:50
For WB: Use at 1:10-1:50
For IHC: Use at 1:10-1:50
For ELISA: Use at 1:20-1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
We recommend heat-mediated antigen retrieval for 15 min in citrate buffer, pH 6.0.