|Application Note||Recommended Starting Dilutions:|
For ICC/IF: Use at a starting dilution of 1:5 - 1:20. Acetone fixation is recommended.
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
For immunostaining cells containing EBNA-1 with clone 0211, slides must be fixed in methanol/acetone (3:7) at -20 °C. After drying at room temperature, slides should be stored at 4°C (1 month) or -20°C (6-12 months). A highly amplified assay must be used to detect the low amount of EBNA expressed, i.e. Monoclonal antibody + Goat Anti-Mouse IgG + Rabbit Anti-Goat IgG-FITC conj. EBNA-1 clones 0211 & 0216 are reactive with the carboxy terminus (~ last 100 aa) of the molecule.