|Application Note||Recommended Starting Dilutions:|
For ICC/IF: Use at 1:10-1:50
For WB: Use at 1:10-1:50
For IHC: Use at 1:10-1:50
For ELISA: Use at 1:20-1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
For immunostaining cells containing EBNA-1 with clone 0211, slides must be fixed in methanol/acetone (3:7) at -20 °C. After drying at room temperature, slides should be stored at 4°C (1 month) or -20°C (6-12 months). A highly amplified assay must be used to detect the low amount of EBNA expressed, i.e. Monoclonal antibody + Goat Anti-Mouse IgG + Rabbit Anti-Goat IgG-FITC conj.