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1 .GTX102732 FAP antibody [N1N3]

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FAP antibody [N1N3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsWB
  • Species reactivityHuman
  • Publication  ( 1 )
Cat No: GTX102732
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-FAP antibody [N1N3] used in Western Blot (WB). GTX102732
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  • Bio-info
  • Protocol & MSDS
  • Application Information: FAP antibody [N1N3]

Catalog Number GTX102732
☆☆☆☆☆
( 0 )
Publication ( 1 )
Product Name FAP antibody [N1N3]
See all FAP products
ApplicationsWB
Application Note
Suggested dilutionReference
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size88 kDa (note) IsoForm (2)
Positive Controls293T , A431 , HepG2 , Molt-4 , Raji
Form SuppliedLiquid
Concentration0.58 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: FAP antibody [N1N3]

Full Namefibroblast activation protein, alpha
BackgroundThe protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq]
SynonymsDKFZp686G13158 antibody, DPPIV antibody, FAPA antibody, FAP antibody, 170 kDa melanoma membrane-bound gelatinase antibody, integral membrane serine protease antibody, seprase antibody, "fibroblast activation protein, alpha antibody"
Cellular LocalizationCell membrane; Single-pass type II membrane protein , Cell projection , lamellipodium membrane; Single-pass type II membrane protein , invadopodium membrane; Single-pass type II membrane protein
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human FAP. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Bovine
Predict Reactivity NoteMouse (89%), Rat (89%), Bovine (93%)
ConjugationUnconjugated

  • Storage Conditions: FAP antibody [N1N3]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Tumor biomarkers
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FAP antibody [N1N3] validated data
  • Anti-FAP antibody [N1N3] used in Western Blot (WB). GTX102732
    • GTX102732 WB Image
      Sample (30 µg of whole cell lysate)
      A: HepG2 (GTX27900)
      B: Molt-4 (GTX27912)
      7.5% SDS PAGE
      GTX102732 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-FAP antibody [N1N3] used in Western Blot (WB). GTX102732
    • GTX102732 WB Image
      Sample (30 µg of whole cell lysate)
      A: HepG2 (GTX27900)
      B: Molt-4 (GTX27912)
      7.5% SDS PAGE
      GTX102732 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
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  • Reference for FAP antibody [N1N3]
Application References Application Reactivity
Wolczyk D et al. TNF-α promotes breast cancer cell migration and enhances the concentration of membrane-associated proteases in lipid rafts. Cell Oncol (Dordr) (Epub ahead of print) (2016). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensFAP2191Q12884, Q53TP5600403NP_00127873684.87
Homo sapiensFAP2191Q12884, Q53TP5600403NM_004460NP_00445187.73

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusFap14089P97321, A2AS87NM_007986NP_03201287.96
Rattus norvegicusFap192203Q8R492NM_138850NP_62020587.86
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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