FBXW2 antibody, C-term
|Applications||IHC, IHC-P, WB|
|Application Note||Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5µg/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both bands were successfully blocked by incubation with the immunising peptide. In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3µg/ml. |
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX25309-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||F-box and WD repeat domain containing 2|
|Product Description||Goat polyclonal antibody to FBXW2|
|Background||F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. A large family of mammalian F-box proteins has recently been identified and classified into three groups based on the presence of either the WD-40 repeats, the leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. The FBXW2 gene product, the second identified member of the F-box gene family, contains multiple WD-40 repeats.|
|Synonyms||Fbox protein Fbw2, F-box and WD repeat domain containing 2, FBW2, FBXW2, Fbox and WD40 domain protein2, F-box and WD-40 domain protein 2, MGC117371, Fwd2, Md6, Fbox and WD40 domain protein 2, Fbox and WD repeat domain containing2, F-box protein Fbw2, Fbox and WD repeat domain containing 2, F box protein Fbw2|
|Specificity||This antibody is specific for FBXW2.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Pig, Bovine|