|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||43 kDa (note)
|Positive Controls||HepG2 , NIH-3T3 , JC , BCL-1 , Raw264.7 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||flap structure-specific endonuclease 1|
|Product Description||Rabbit Polyclonal antibody to FEN1 (flap structure-specific endonuclease 1)|
|Background||The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq]|
|Synonyms||FEN-1 antibody, MF1 antibody, RAD2 antibody, FEN1 antibody, flap endonuclease 1 antibody, hFEN-1 antibody, maturation factor-1 antibody, DNase IV antibody, maturation factor 1 antibody, flap structure-specific endonuclease 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Chicken, Sheep, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Chicken (81%), Sheep (94%), Rhesus Monkey (98%), Bovine (96%)|