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1 .GTX28420 FLASH antibody

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FLASH antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse
Cat No: GTX28420
Package Price Qty
100 μgUSD 349.00
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  • Anti-FLASH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28420
  • Anti-FLASH antibody used in Western Blot (WB). GTX28420
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  • Datasheet

  • Application Information: FLASH antibody

Catalog Number GTX28420
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Product Name FLASH antibody
See all FLASH products
ApplicationsICC/IF, WB
Application NoteFor WB: Use at a dilution of 1:1,000-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size223 kDa (note)
Positive ControlsHeLa cell lysate
Form SuppliedLiquid
Concentration0.5 mg/ml (Please refer to the vial label for the specific concentration)
PurificationAffinity purified with antigen

  • Specifications: FLASH antibody

Full Namecaspase 8 associated protein 2
BackgroundA novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
SynonymsCASP8AP2, KIAA1315, 606880, CED-4, CED4, FLASH, Q9UKL3, RIP25, FLJ11208, 9994, CED 4
HostRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen1221~1270a.a of human FLASH(AAD45157). This sequence differs by one amino acid from that of mouse.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
ConjugationUnconjugated

  • Storage Conditions: FLASH antibody

Storage BufferPhosphate-buffered saline, pH 7.4 containing 0.02% sodium azide
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Apoptosis > Induction > Death domain receptors
  • Cancer > Apoptosis > Induction > Extracellular signals
  • Cell Biology > Apoptosis > Induction from death receptors
  • Cell Biology > Apoptosis > Induction from extracellular signals
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FLASH antibody validated data
  • Anti-FLASH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28420
    • GTX28420 ICC/IF Image
      Immunocytochemical staining of HeLa cells with Rabbit anti Human FLASH (GTX28420)
      Top
  • Anti-FLASH antibody used in Western Blot (WB). GTX28420
    • GTX28420 WB Image
      Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/ml.
      Top
  • Anti-FLASH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28420
    • GTX28420 ICC/IF Image
      Immunocytochemical staining of HeLa cells with Rabbit anti Human FLASH (GTX28420)
      Top
  • Anti-FLASH antibody used in Western Blot (WB). GTX28420
    • GTX28420 WB Image
      Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/ml.
      Top
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